北京师范大学学报（自然科学版）2017，Vol.53Issue(2)：170-174,5.DOI:10.16360/j.cnki.jbnuns.2017.02.010
大肠杆菌对氮限制的适应性进化
Evolutionary adaptation of Escherichia coli to nitrogen limitation
摘要
Abstract
Organisms often face nitrogen limitation in the natural environment and they can adapt to it through different ways.Comparisons of existing taxa suggest that organisms tend to adapt to nitrogen limitation by nitrogen conservation,but evolutionary experiments suggest that increased nitrogen acquisition led to adaptation.These two types of studies have methodological drawbacks.Therefore we designed a more synthetic evolutionary experiment.We used Escherichia coli to evolve in two different nitrogen environments for 1 300 generations and analyzed their adaptation to these environments.After 1 300 generations,the fitness of all evolved lines in the two environments was significantly increased.The lines evolved in low nitrogen environment had more fitness gains than the lines evolved in high nitrogen environment.Compared with their ancestors,the lines evolved in low nitrogen environment showed significantly increased N％ and DNA％,but decreased biomass C∶ N ratio.While lines evolved in high nitrogen environment showed significantly increased RNA％.These data suggest that E.coli adapts to nitrogen limitation by stronger ability to acquire nitrogen but not nitrogen conservation.关键词
实验进化/相对适合度/氮限制Key words
experimental evolution/relative fitness/nitrogen limitation分类
生物科学引用本文复制引用
倪川,朱璧如,姜新华,张大勇..大肠杆菌对氮限制的适应性进化[J].北京师范大学学报（自然科学版）,2017,53(2):170-174,5.基金项目
国家自然科学基金资助项目(31030014) （31030014）
