重庆理工大学学报（自然科学版）2017，Vol.31Issue(5)：99-104,147,7.DOI:10.3969/j.issn.1674-8425(z).2017.05.017
禽流感H9N2病毒样颗粒疫苗佐剂的筛选及其对鸡的免疫效果评价
Screening and Immune Protection Evaluation of Different Adjuvanted Avian Influenza H9N2 Virus-Like Particle Vaccines in Chicken
摘要
Abstract
In order to have effectively prevention and control of avian influenza H9N2 pandemic, three kind of adjuvanted avian influenza H9N2 virus-like particle vaccines (oil-emulsion,ISA 206 and microspheres) were developed and selected to get the best one for avian influenza H9N2 VLPs by immunity evaluation in chicken.The HA assay was performed to demonstrate the agglutination activity of VLPs and the HA protein content was evaluated by the standard quantification method that is indirect ELISA assay.Then the stability, sterility and security of H9N2 VLPs vaccines were tested before vaccination.HI assay was used to assess the functional antibodies in the sera of immunized chicken to HA.Determination of T lymphocyte stimulation index and lysozyme content were investigated by MTT and lysozyme content detection kit.The results show that chicken orally vaccinated with the microspheres H9N2 VLPs generated higher lysozyme content and HI titers of antibody against H9N2 viruses than other groups(p<0.01) and little or no cellular immune response.The microspheres H9N2 VLPs vaccines provided the best safety, sterility and immunogenicity to chicken and these results indicate a reference for the choice of adjuvanted avian influenza H9N2 VLPs vaccines.关键词
H9N2禽流感病毒/病毒样颗粒/佐剂/微球Key words
avian influenza H9N2/virus-like particle/adjuvant/microsphere分类
农业科技引用本文复制引用
周康森,顾盼,王玲玲,吴胜昔,蔡家利..禽流感H9N2病毒样颗粒疫苗佐剂的筛选及其对鸡的免疫效果评价[J].重庆理工大学学报（自然科学版）,2017,31(5):99-104,147,7.基金项目
重庆高校优秀成果转化资助项目(KJZH14212) （KJZH14212）
