大豆科学2017，Vol.36Issue(3)：441-446,6.DOI:10.11861/j.issn.1000-9841.2017.03.0441
大豆种质资源对尖镰孢菌根腐病的抗性鉴定及生理分析
Identification for Resistance to Root Rot Caused by Fusarium Oxysporum in Soybean Germplasm and Physiological Analysis
摘要
Abstract
In the present study,a total of 342 soybean germplasm from 19 provinces and foreign countries were evaluated for the resistance to F.Oxysporum with the method of changed bacteria layer inoculation.The 19 provinces mainly contain 95 resources from Heilongjiang,21 resources from Jilin,19 resources from Liaoning,6 resources from Inner Mongolia,23 resources from Beijing,12 resources from Hebei,3 resources from Anhui and 24 resources from American.SOD,soluble sugar,and MDA were studied after seeding root of resistant and susceptible soybean were treated by microbial from F.Oxysporum.In order to lay the foundation for molecular assisted breeding.The appraisal result showed that ten soybean accessions with high resistance to F.Oxysporum were identified and none accession were found to be immune to F.Oxysporum.The result of source showed that resistance resources from the north are richer than the south.The result of biochemical showed that after inoculated by microbial in two varieties.The activity of SOD raised first and downward trend.The content of soluble sugar decreased and they were much higher in resistant variety than in susceptible one,the change of MDA in resistant obviously smaller than the susceptible one.关键词
大豆根腐病/尖镰孢菌/抗病鉴定/病情指数/生化指标Key words
Soybean root rot/Fusarium Oxysporum/Identification for resistance/Disease index/Biochemical index分类
农业科技引用本文复制引用
张婵娟,廖胜泉,宋欢,赵雪,韩英鹏,刘琦,李文滨,武小霞..大豆种质资源对尖镰孢菌根腐病的抗性鉴定及生理分析[J].大豆科学,2017,36(3):441-446,6.基金项目
国家自然科学基金(31301339) （31301339）
黑龙江省教育厅科学技术研究项目(12541049) （12541049）
黑龙江省教育厅新世纪项目优秀人才项目资助(1253-NCET-005) （1253-NCET-005）
教育部博士点项目(20122325120012) （20122325120012）
黑龙江省自然科学基金面上项目(C201130). （C201130）
