大连工业大学学报2017，Vol.36Issue(3)：162-167,6.
仿刺参溶菌酶在重组酿酒酵母中的表达
Expression of Apostichopus japonicas lysozyme in recombinant Saccharomyces cerevisiae
摘要
Abstract
Apostichopus japonicus lysozyme (AjLys) was expressed by Saccharomyces cerevisiae.The His-tag was inserted into the N terminus of AjLys gene based on pMD18-AjLys vector to construct the new plasmid pMD18-His6-AjLys.According to the analysis by Codon Usage Database in S.cerevisiae, AjLys gene was optimized by point mutation to obtain pMD18-His6-AjLys (Δ69, 77, 95) vector.The recombinant expression vectors pESC-LEU-His6-AjLys and pESC-LEU-His6-AjLys (Δ69, 77, 95) were constructed.AjLys protein was successfully expressed in the recombinant pESC-LEU-His6-AjLys (Δ69, 77, 95)/MKP-0 cell inducted by galactose, which was detected by Western Blot.The expressed AjLys protein displayed inhibitive effect on the growth of the Micrococcus lysodeikticus.The result showed that AjLys expression vector of S.cerevisiae was constructed successfully, and the active AjLys protein was expressed in MKP-0 cells, which provided a new method for the production of AjLys.关键词
仿刺参/溶菌酶/酿酒酵母/密码子优化/表达Key words
Apostichopus japonicus/lysozyme/Saccharomyces cerevisiae/codon optimization/expression分类
轻工纺织引用本文复制引用
随瑞瑞,沈咪娜,李成,赵钰,车明月,张齐,丛丽娜..仿刺参溶菌酶在重组酿酒酵母中的表达[J].大连工业大学学报,2017,36(3):162-167,6.基金项目
辽宁省教育厅一般科学研究项目(L2014217) （L2014217）
辽宁省教育厅重点实验室项目(LZ2014029) （LZ2014029）
辽宁省研究生教育创新计划项目(2014-154). （2014-154）
