动物营养学报2017，Vol.29Issue(6)：1895-1903,9.DOI:10.3969/j.issn.1006-267x.2017.06.010
金华猪和长白猪粪便微生物移植小鼠的肠道古菌结构分析
Intestinal Archaea Community Structure Analysis of Mice Transplanted with Jinhua and Landrace Pig Feces
摘要
Abstract
This experiment was conducted to explore the differences of the intestinal archaea community structures between the obese breed Jinhua pig and the lean breed Landrace pig,and to investigate the changes of the archaea community structure during the inter-species fecal microbiota transplantation (FMT).The fresh feces of Jinhua and Landrace pigs were collected and introduced into broad spectrum antibiotics treated germ-free mice guts by gavage,respectively.The total genomic DNA was extracted from the pig fecal samples and the transplanted mice cecal contents,and was used as templates to amplify the V4 region of the 16S rRNA gene by PCR with the universal primers of archaea.The PCR products were then subjected to high-throughput sequencing on an Illumina HiSeq sequencing platform. The obtained sequences were subsequently processed and analyzed with a series of software such as QIIME.The results showed as follows: at the phylum level,three main phyla of archaea,Thaumarchaeota,Euryarchaeota,and Crenarchaeota,were found in pig fecal samples and transplanted mice cecal contents,with Thaumarchaeota as the dominant phylum;while at the genus level,five known genera of archaea were observed,with Cenarchaeum as the dominant genus.The abundance of phylum Euryarchaeota in Jinhua pig's feces was higher than that in Landrace pig's feces.Thaumarchaeota dominate the archaea community structure of transplanted mice cecal contents,with abundance of more than 70%,and Thaumarchaeota mainly consisted of Cenarchaeum.It was observed that no significant difference was exhibited between the two groups of mice at the phylum and genus levels (P>0.05),but the operational taxonomic unit (OTU) based principal coordinate analysis showed a good aggregation within each group.It is concluded that genus Cenarchaeum of phylum Thaumarchaeota is the dominant archaea in feces of Jinhua and Landrace pigs and cecal contents of mice recipients.Phylum Euryarchaeota is more abundant in Jinhua pig's feces than in Landrace pig's feces, and there is difference in archaea community structures between their respective mice recipients at the OTU level.关键词
古菌/金华猪/长白猪/粪菌移植/高通量测序Key words
archaea/Jinhua pig/Landrace pig/fecal microbiota transplantation/high-throughput sequencing分类
农业科技引用本文复制引用
肖英平,王军军,李天天,唐标,何祥祥,李开锋,杨华..金华猪和长白猪粪便微生物移植小鼠的肠道古菌结构分析[J].动物营养学报,2017,29(6):1895-1903,9.基金项目
浙江省自然科学基金重点项目(LZ15C170001) （LZ15C170001）
北京食品营养与人类健康高精尖创新中心开放基金 （）
国家自然科学基金(31402083) （31402083）
