动物营养学报2017，Vol.29Issue(6)：1940-1948,9.DOI:10.3969/j.issn.1006-267x.2017.06.015
豆油部分或全部替代饲料中鱼油对中华条颈龟肠道形态及菌群结构的影响
Effects of Partial or Total Replacement of Fish Oil by Soybean Oil on Intestinal Morphology and Microbiota Structure of Chinese Striped-Neck Turtle (Mauremys sinensis)
摘要
Abstract
This study was conducted to investigate the effects of partial or total replacement of fish oil by soybean oil on intestinal morphology and microbiota structure of Chinese striped-neck turtle (Mauremys sinensis) by histological staining and 16S rRNA V3-V4 region sequencing.Twenty 4-year adult female Chinese striped-neck turtles were randomly divided into 4 groups and each group had 6 turtles.Turtles in group Ⅰ (control group) were fed a formula feed containing 1% fish oil which was a whole fish oil diet with the ratio of soybean oil to fish oil was 0∶3, and others in three experimental groups were fed diets used soybean oil to replace 33% (groupⅡ), 67% (group Ⅲ) and 100% (group Ⅳ) fish oil based on the whole fish oil diet and the ratios of soybean oil to fish oil of them were 1∶2, 2∶1 and 3∶0, respectively.Turtles were fed 2 times per week, and the experiment lasted for 10 months.The results showed as follows: 1) the ratio of villus height to crypt depth (VH/CD) of duodenum in groups Ⅰ and Ⅲ was significantly higher than that in groups Ⅱ and Ⅳ (P<0.05).2) According to the 97% level of sequence similarity, the total numbers of operational taxonomic unit (OTU) in groupsⅠ, Ⅱ, Ⅲ and Ⅳ were 396, 321, 347 and 331, while the specific OTU numbers were 43, 3, 4 and 5, and 139, 117, 128, 120 genus were identified, respectively.The dominant phyla in the duodenum were Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria.The proportions of Fusobacteria and Proteobacteriai were increased while the proportions of Bacteroidetes and Firmicutes were decreased in groups Ⅱ, Ⅲ and Ⅳ.However, the small gap of Firmicutes and Bacteroidetes between groups Ⅰ and Ⅲ.The bacterial Ace index and Shanno index were the highest in group Ⅰ, the middle in groups Ⅲ and Ⅳ, and the lowest in group Ⅱ.The bacterial Simpson index was the highest in group Ⅱ, the middle in groups Ⅲ and Ⅳ, and the lowest in group Ⅰ.Therefore, the development maturation degree of intestinal cells and intestinal microbiota structure of Chinese striped-neck turtle fed the diet with 67% fish oil replacement by soybean oil are similar to Chinese striped-neck turtle fed the whole fish oil diet.关键词
中华条颈龟/鱼油替代/肠道形态/肠道菌群结构Key words
Chinese striped-neck turtle (Mauremys sinensis)/replacement of fish oil/intestinal morphology/intestinal microbiota structure分类
农业科技引用本文复制引用
丁利,李江月,薛珊,史海涛,洪美玲..豆油部分或全部替代饲料中鱼油对中华条颈龟肠道形态及菌群结构的影响[J].动物营养学报,2017,29(6):1940-1948,9.基金项目
国家自然科学基金项目(31360642,31372228) （31360642,31372228）
海南省大学生创新训练项目(3010151072) （3010151072）
