动物营养学报2017，Vol.29Issue(6)：1988-1995,8.DOI:10.3969/j.issn.1006-267x.2017.06.020
表皮生长因子调控猪肠上皮细胞中钠依赖Ⅱb型磷转运蛋白表达的细胞信号通路研究
Identifying Cell Signaling Pathway that Mediates Epidermal Growth Factor Regulating Sodium Dependent Phosphate Cotransporters Type Ⅱb Expression in Porcine Intestinal Epithelial Cells
摘要
Abstract
The aim of this study was to elucidate the molecular mechanisms of epidermal growth factor (EGF) down-regulated sodium dependent phosphate cotransporters type Ⅱb (NaPi-Ⅱb) expression in porcine intestinal epithelial cells (IPEC-J2).EGF receptor tyrosine kinase inhibitor (tyrphostin AG 1478), protein kinase A (PKA) inhibitor (H89), protein kinase C (PKC) inhibitor (k4393), p38 inhibitor (SB 203580), extracellular signal regulated kinase (ERK) inhibitor (PD98059) and c-Jun n-terminal kinase (JNK) inhibitor (anisomycin) as well as EGF were used to treat IPEC-J2 cells, respectively, and related signal pathways protein and interest protein (NaPi-Ⅱb) expression levels were determined by Western blot.The results showed as follows, compared with control group, NaPi-Ⅱb expression level was significantly decreased after EGF treatment (P<0.05).Compared with none inhibitor treatments, inhibitors of EGF receptor, PKA, PKC, mitogen activated protein kinase (MAPK)/p38, MAPK/ERK1/2 and MAPK/JNK significantly increased NaPi-Ⅱb expression (P<0.05).Especially, MAPK/ERK1/2 inhibitor significantly reduced the phosphorylation level of MAPK/ERK1/2 at Tyr204 site (P<0.05), and MAPK/JNK inhibitor significantly reduced the phosphorylation level of MAPK/JNK1/2/3 at Thr183 and Tyr185 sites (P<0.05).In summary, all EGF receptor, PKA, PKC, p38, ERK and JNK participate in the inhibitory effects of EGF on NaPi-Ⅱb expression.关键词
表皮生长因子/磷/NaPi-Ⅱb/信号通路/猪小肠上皮细胞Key words
epidermal growth factor/phosphate/NaPi-Ⅱb/signal pathway/porcine intestinal epithelial cells分类
农业科技引用本文复制引用
邢廷杰,汤小朋,曹满湖,方热军..表皮生长因子调控猪肠上皮细胞中钠依赖Ⅱb型磷转运蛋白表达的细胞信号通路研究[J].动物营养学报,2017,29(6):1988-1995,8.基金项目
国家自然科学基金面上项目(31572419) （31572419）
长沙市科技计划项目(kh1601185) （kh1601185）
