动物营养学报2017，Vol.29Issue(6)：1996-2009,14.DOI:10.3969/j.issn.1006-267x.2017.06.021
血红素铁对妊娠母鼠繁殖成绩及组织铁调基因表达的影响
Effects of Heme Iron on Reproductive Performance and Iron-Regulated Gene Expressions in Tissues of Pregnant Mice
摘要
Abstract
This study was conducted to compare the effects of heme iron (Fe) and ferrous sulfate (FeSO4) on reproductive performance, the Fe content in pregnant mice tissues and fetal mice, and the expressions of Fe-regulated genes in tissues of pregnant mice.Eighty Kunming female mice with similar body condition at 2 months of age were selected and randomly divided into 8 groups with 10 mice per group.The groups were control group, Fe deficiency group, heme Fe (15, 60 and 90 mg/kg) groups and FeSO4 (75, 300 and 450 mg/kg) groups.Since pregnant, control group was fed normal diet (a basal diet supplemented with 400 mg/kg FeSO4);the other groups were fed the basal diet, and were injected 40 mg/kg deferoxamine to induce Fe deficiency model at 10 to 13 d of pregnant;heme Fe and FeSO4 were supplemented in heme Fe groups and FeSO4 groups from 14 d of pregnant, while none of them was supplemented in Fe deficiency group.The experiment was carried out at 1 to 20 d of pregnant.The results showed as follows: 1) the weight of fetal mice in 60 mg/kg group was significantly higher than that in control group and Fe deficiency group (P<0.01).2) Blood hemoglobin (HGB) content, red blood cell (RBC) and hematocrit (HCT) in 60 mg/kg heme Fe group and 450 mg/kg FeSO4 group were significantly higher than those in Fe deficiency group (P<0.01).3) The Fe content in the fetal mice in 60 mg/kg heme Fe group was significantly higher than that in control group and FeSO4 groups (P<0.01);the Fe content in liver, spleen and placenta was the highest in450 mg/kg FeSO4 group.4) The expression of hepcidin in liver of pregnant mice was higher in 90 mg/kg heme Fe group and 450 mg/kg FeSO4 group, which was significantly higher than that in Fe deficiency group and control group (P<0.01);the expressions of Fpn and Tfr2 in liver in 15 mg/kg heme Fe group were higher, which were significantly higher than those in Fe deficiency group and control group (P<0.01);the expressions of Tfr1 and Flvcr in liver in 60 mg/kg heme Fe group were higher, which were significantly higher than those in Fe deficiency group and control group (P<0.01);the expression of Tfr1 in liver in 75 mg/kg FeSO4 group was higher, which was significantly higher than that in Fe deficiency group and control group (P<0.01).5) Fpn, HCP, DMT1 and Flvcr expressions in duodenum of pregnant mice in Fe deficiency group were significantly higher than those in control group (P<0.01).6) Placenta Fpn, HCP, DMT1, Flvcr and Tfr1 expressions were significantly higher than those in control group (P<0.01);the expression of hepcidin in placenta in 90 mg/kg heme Fe and 450 mg/kg FeSO4 was higher, which was significantly higher than that in Fe deficiency group and control group (P<0.01).7) The Fe content of fetal mice was the highest when heme Fe was 61.00 mg/kg or FeSO4 was 336.11 mg/kg;the Fe content in liver of pregnant mice was the highest when heme Fe was 93.49 mg/kg.In conclusion, the supplementation of heme Fe and FeSO4 at proper levels can increase the weight of fetal mice, regulate the expressions of Fe-regulating genes in target tissues of pregnant mice, and increase the body Fe content of pregnancy mice and 20 d fetal mice;HCP and Flvcr play critical roles in the intestinal absorption or placental transport of heme Fe, while intestinal absorption and placental transport of FeSO4 mainly rely on DMT1 and Tfr2.关键词
妊娠母鼠/血红蛋白/铁含量/铁调素/血红素铁Key words
pregnant mice/hemoglobin/iron content/hepcidin/heme iron分类
农业科技引用本文复制引用
李美荃,张春勇,计乔平,陈克嶙,郭荣富..血红素铁对妊娠母鼠繁殖成绩及组织铁调基因表达的影响[J].动物营养学报,2017,29(6):1996-2009,14.基金项目
云南省重大科技计划--生物育种(2012ZA018-3) （2012ZA018-3）
