南方医科大学学报2017，Vol.37Issue(6)：730-736,7.DOI:10.3969/j.issn.1673-4254.2017.06.03
甲基化转移酶抑制剂BIX01294促进小鼠子宫基质细胞迁移、抑制基质细胞蜕膜化
Methyltransferase inhibitor BIX01294 promotes the migration and inhibits decidualization of mouse uterine stromal cells in vitro
摘要
Abstract
Objective To investigate the effect of BIX01294 (BIX), a methyltransferase inhibitor, on the migration and decidualization of the stromal cells in mouse uterus. Methods Mouse endometrial stromal cells were isolated and cultured from the uterus of pregnant mice on day 3.5 of gestation. The migration and decidualization of mouse endometrial stromal cells treated with BIX at different concentrations were observed with wound healing assay and real-time PCR. Results The migration distance of mouse endometrial stromal cells increased as the BIX concentration increased within the range below 15 μmol/L. Compared with the control cells, the cells treated with BIX (15 μmol/L) showed significantly increased migration distances, but increasing BIX concentration to 20 μmol/L did not further increase the cell migration distance and began to cause cell death. Compared with the control cells, the BIX-treated stromal cells exhibited significantly down-regulated expression of Ehmt2 mRNA, and 15 μmol/L BIX caused inhibition of decidualization in the stromal cells. Conclusion Within a defined concentration range, BIX promotes the migration and inhibits decidualization of mouse uterine stromal cells by inhibiting the expression of Ehmt2 mRNA.关键词
子宫内膜基质细胞/迁移/蜕膜化/BIX01294/组蛋白甲基化Key words
endometrial stromal cells/cell migration/decidualization/BIX01294/histone methylation引用本文复制引用
廖晖淇,田鎏,杨慧,马妮,张昌军,刁红录..甲基化转移酶抑制剂BIX01294促进小鼠子宫基质细胞迁移、抑制基质细胞蜕膜化[J].南方医科大学学报,2017,37(6):730-736,7.基金项目
国家自然科学基金(31671565) （31671565）
湖北省自然科学基金(2015CFB543) （2015CFB543）
湖北医药学院创新团队(2014CXX03,FDFR201604) （2014CXX03,FDFR201604）
湖北医药学院重点学科建设项目资助项目Supported by National Natural Science Foundation of China (31671565). （31671565）
