南方医科大学学报2017，Vol.37Issue(6)：761-766,6.DOI:10.3969/j.issn.1673-4254.2017.06.08
3D生物打印的微结构促进小鼠表皮干细胞的增殖和活性
Three-dimensional bioprinted microstructure promotes proliferation and viability of murine epithelial stem cells in vitro
摘要
Abstract
Objective To evaluate the effect of different microstructures prepared by three-dimensional (3D) bioprinting on proliferation and viability of the murine epithelial stem cells in vitro. Methods 3D cell-laden microstructures were constructed using 3 different printing nozzles with diameters of 210, 340, and 420 μm. Fluorescence microscopy and the live/dead assay kit were used to observe the proliferation and viability of the murine epithelial stem cells in the microstructures. Results All the 3D cell-laden micro-structures were capable of promoting the proliferation of murine epithelial stem cells. In the 3 groups of micro-structures, the cell viability decreased significantly with time until 7 days after printing (P<0.01), but at 14 days after the printing, the cell viability increased significantly as compared with that at 7 days (P<0.01). The viability of the cells was significantly higher in the microstructure printed using a 420 μm nozzle than in the microstructures printed with 210 μm and 340 μm nozzles (P<0.01). Conclusion The microstructure printed with a 420 μm nozzle can stably promote the proliferation of murine epithelial stem cells and maintain a high level of cell viability, suggesting the feasibility of constructing tissue-engineered epidermis and full-thickness skin graft using 3D bioprinting technique.关键词
3D生物打印技术/3D微结构/表皮干细胞/细胞增殖/细胞活性Key words
three-dimensional bioprinting/three-dimensional microstructures/epithelial stem cells/cell proliferation/cell viability引用本文复制引用
刘煜凡,黄沙,姚斌,李曌,李想,付小兵,吴旭..3D生物打印的微结构促进小鼠表皮干细胞的增殖和活性[J].南方医科大学学报,2017,37(6):761-766,6.基金项目
国家重点基础研究发展计划"973计划"(2012CB518105) （2012CB518105）
国家自然科学基金(81121004,81671905) Supported by National Key Basic Research Program (2012CB518105) and Supported by National Natural Science Foundation of China (81121004, 81671905). （81121004,81671905）
