南方医科大学学报2017，Vol.37Issue(6)：774-779,6.DOI:10.3969/j.issn.1673-4254.2017.06.10
血清限制对人滋养细胞株HTR-8/SVneo细胞胰岛素样生长因子-1表达及细胞侵袭力的影响
Effect of serum restriction on insulin like growth factor-1 expressions and invasiveness in human trophoblast HTR-8/SVneo cells in vitro
摘要
Abstract
Objective To explore the effect of serum restriction on the invasiveness and expressions of insulin-like growth factor-1 (IGF-1) and matrix metalloproteinase-2 (MMP-2) in human trophoblast HTR-8/SVneo cells in vitro. Methods HTR-8/SVneo cells were cultured in the presence of 1%, 5%, or 10%fetal bovine serum (FBS) for 48 h. Fluorescence quantitative PCR and immunofluorescence staining were employed to examine the changes in IGF-1 and MMP-2 expressions at both the mRNA and protein levels in HTR-8/SVneo cells;MTT assay and Transwell invasion assay were used to assess the changes of the cell proliferation and the cell invasion ability, respectively. MMP-2 expression, cell proliferation and invasiveness were also assessed in the cells treated with recombinant human IGF-1. Results HTR-8/SVneo cells exhibited significantly lowered cell proliferation in cultures containing low concentrations of FBS (P<0.05). The expressions of IGF-1 and MMP-2 at both mRNA and protein levels were significantly down-regulated and the invasiveness was significantly lowered in cells cultured in the medium containing 1%FBS as compared with those of cells cultured in the presence of 5%and 10%FBS (P<0.05). Treatment of the cells with recombinant human IGF-1 significantly up-regulated MMP-2 expression (P<0.05) and increased the cell invasiveness (P<0.05). Conclusions FBS restriction down-regulates IGF-1 expression in human trophoblast HTR-8/SVneo cells and suppress the cell invasiveness possibly by suppressing MMP-2 expression. Treatment with recombinant human IGF-1 can up-regulate MMP-2 expression and promote the invasiveness of HTR-8/SVneo cells.关键词
滋养细胞/血清限制/胰岛素样生长因子-1/基质金属蛋白酶-2/侵袭Key words
trophoblasts/serum restriction/insulin like growth factor-1/matrix metalloproteinase-2/invasiveness引用本文复制引用
周尚谦,沈朗,李雪媛,谢晓珍,芮塬,陈年坤,王志坚..血清限制对人滋养细胞株HTR-8/SVneo细胞胰岛素样生长因子-1表达及细胞侵袭力的影响[J].南方医科大学学报,2017,37(6):774-779,6.基金项目
广东省科技计划项目(2015B010106002) （2015B010106002）
