南方医科大学学报2017，Vol.37Issue(6)：786-791,6.DOI:10.3969/j.issn.1673-4254.2017.06.12
变异链球菌UA159中SMU.2055基因在致龋性的作用
Role of SMU.2055 gene in cariogenic capacity of Streptococcus mutans
摘要
Abstract
Objective To construct a SMU.2055-dificient mutant strain of Streptococcus mutans (S. mutans) and evaluate its cariogenic capacity in comparison with wild-type S. mutans. Methods The SMU.2055-dificient mutant strain of S. mutans was constructed using homologous recombination technique and observed with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The absorbance at 600 nm and pH values of the wild-type and mutant strains were monitored to evaluate their growth and acid production. After acid adaption, the two strains were challenged with acid shock and their survival rates were determined. Results PCR and sequence analyses verified the successful construction of the SMU.2055-dificient mutant strain. Observation with SEM revealed obvious changes in the morphology of the mutant strain, which showed reduced irregular substances between the individual bacteria as compared with the wild-type strain. TEM revealed major alterations in the cellular architecture of the mutant strain with blurry cell membrane and disruption of the membrane integrity. The growth capacity of the mutant strain decreased in both normal and acidic conditions but its acid production capacity remained unaffected. Conclusion SMU.2055 gene is associated with morphology maintenance, growth capacity and acid resistance of S. mutans but is not related to the acid production capacity of the bacterium.关键词
变异链球菌/乙酰基转移酶/SMU.2055基因/致龋性Key words
Streptococcus mutans/acetylase/SMU.2055 gene/cariogenic引用本文复制引用
陈璇,许晓虎,吴昕彧,李转玲,赵望泓..变异链球菌UA159中SMU.2055基因在致龋性的作用[J].南方医科大学学报,2017,37(6):786-791,6.基金项目
南方医科大学南方医院院长基金(2013B003) （2013B003）
深圳市科技计划项目(JCYJ20160428142231354) （JCYJ20160428142231354）
