南方医科大学学报2017，Vol.37Issue(6)：802-806,5.DOI:10.3969/j.issn.1673-4254.2017.06.15
不同pH条件下高低致龋性变异链球菌sRNA SpR19及其潜在靶标GroEL的表达变化
Changes in expressions of sRNA SpR19 and its potential target GroEL in Streptococcus mutans strains with different cariogenicity cultured under different pH conditions
摘要
Abstract
Objective To investigate the changes in the expression level of sRNA SpR19 and its potential target protein GroEL in clinical isolates of Streptococcus mutans with different cariogenicity exposed to different pH conditions and explore the possibility of using these molecules as biomarkers for assessing the cariogenicity of the bacteria. Methods The total RNAs were extracted from the clinical isolates of Streptococcus mutans with high (strain 17) and low cariogenicity (strain 5) for high-throughput sequencing for profiling of the differentially expressed sRNAs. The candidate sRNA, SpR19, was selected for further study on the basis of bioinformatics analysis considering the role of its potential target in the cariogenic process. The differential expression levels of SpR19 in the strains exposed to both pH5.5 and pH7 culture conditions were verified by quantitative real-time PCR. The expression of the potential target of SpR19, GroEL, was also investigated at both the protein and mRNA level using Western blotting and quantitative real-time PCR. Results Bioinformatic analysis suggested multiple potential target sites of SpR19 both in GroEL mRNA and in the upstream and downstream inter-genic regions. Under different pH conditions, the highly cariogenic strain 17 expressed consistently low levels of SpR19 as compared with the strain 5 with a low cariogenicity;GroEL showed a reverse expression pattern in the 2 strains. An inverse correlation was found between the expressions of SpR19 and GroEL. Conclusion The highly cariogenic strain 17 expressed low levels of SpR19 and high levels of GroEL in both acidic and neutral culture conditions. SpR19 may negatively regulate the cariogenicity of Streptococcus mutants by targeting at GroEL.关键词
变异链球菌/GroEL/sRNASpR19/致龋Key words
Streptococcus mutans/GroEL/SpR19/cariogenicity引用本文复制引用
胡桐楠,郑伟,李少华,董洁,王心玲,王成龙,邵宁生,储冰峰..不同pH条件下高低致龋性变异链球菌sRNA SpR19及其潜在靶标GroEL的表达变化[J].南方医科大学学报,2017,37(6):802-806,5.基金项目
军队十二五面上项目(CWS12J126) （CWS12J126）
