南方医科大学学报2017，Vol.37Issue(6)：827-832,6.DOI:10.3969/j.issn.1673-4254.2017.06.20
化香树果序乙醇提取物诱导CNE1、CNE2细胞发生methuosis死亡的机制
Mechanism of Platycarya strobilacea Sieb. et Zucc extract-induced methuosis in human nasopharyngeal carcinoma CNE1 and CNE2 cells
摘要
Abstract
Objective To study the effect of Platycarya strobilacea Sieb. et Zucc (PSZ) extract on methuosis of human nasopharyngeal carcinoma CNE1 and CNE2 cells and explore the underlying mechanism. Methods CNE1 and CNE2 cells were treated with 1 mg/mL PSZ extract and the expressions of Rac1 mRNA and Rac1 protein were detected using RT-qPCR and Western blotting, respectively. Results CNE1 and CNE2 cells showed obvious morphological changes typical of methuosis following treatment with PSZ extract characterized by cell merging, accumulation of large cytoplasmic vacuoles, and membrane rupture without obvious changes in the nuclei. PSZ treatment resulted in up-regulated Rac1 mRNA and Rac1 protein expressions in the cells. Application of EHT 1864 obviously blocked the effect of PSZ extract in inducing methuosis in CNE1 and CNE2 cells. Conclusion PSZ extract can induce methuosis in CNE1 and CNE2 cells by inducing the overexpression of Rac1.关键词
鼻咽癌/化香树果序乙醇提取物/methuosis/Rac1Key words
nasopharyngeal carcinoma/Platycarya strobilacea Sieb. et Zucc/methuosis/Rac1引用本文复制引用
朱俊谕,涂维,曾超,毛珩旭,杜庆锋,蔡红兵..化香树果序乙醇提取物诱导CNE1、CNE2细胞发生methuosis死亡的机制[J].南方医科大学学报,2017,37(6):827-832,6.基金项目
广东省自然科学基金(2016A030313724) （2016A030313724）
广东省中医药局科研项目(20173028) （20173028）
