南方医科大学学报2017，Vol.37Issue(6)：837-841,5.DOI:10.3969/j.issn.1673-4254.2017.06.22
等候肾移植患者中RHD阴性血液样品的基因分型及意义
Genotyping of RhD-negative blood samples diagnosed by serological tests from patients waiting for kidney transplantation
摘要
Abstract
Objective To compare the accuracy of serological and molecular approaches to identification of RhD-negative patients waiting for kidney transplantation. Methods A total of 103 RhD-negative blood samples by serological test were collected from patients waiting for kidney transplantation between January, 2006 and January, 2016. Quantitative PCR and sequencing were used to verify the results of RHD genotyping, and the false negative rates of the serological and molecular methods for RhD genotyping were compared. Results Among the 103 blood samples, true RhD negativity (with all the 10 exons missing) was found in 56 samples (54.5%), and false RhD negativity (RhD positivity with loss, repetition, or missense mutation in the 10 exons) in 47 samples (45.6%). In the 47 false RhD-negative cases, weak D was detected in 1 case (2.1%), partial D in 13 cases (27.7%), and D-elution in 33 cases (70.2%). The detection rates of RhD negativity differed significantly between the serological and molecular methods (P<0.05). Conclusion Serological test is associated with a high false negative rate in detecting RhD blood group, and the use of the molecular approach has important clinical significance in accurate RhD genotyping for patients waiting for renal transplantation.关键词
RhD血型阴性/肾移植/分子生物学Key words
RhD blood group/kidney transplantation/polymerase chain reaction,real time引用本文复制引用
付绍杰,奉艳林,于立新,苗芸,罗敏,王亦斌,李怿辰,陈树汉,肖露露..等候肾移植患者中RHD阴性血液样品的基因分型及意义[J].南方医科大学学报,2017,37(6):837-841,5.基金项目
广东省科技计划项目(2014A020212582) （2014A020212582）
