分析测试学报2017，Vol.36Issue(4)：519-523,5.DOI:10.3969/j.issn.1004-4957.2017.04.013
L-苏氨酸分子印迹传感器的制备及应用
Preparation of a Molecularly Imprinted Film Modified Electrochemical Sensor for L-threonine Determination
摘要
Abstract
A nano-silver/poly quercetin composites modified paraffin-impregnated graphite electrode was prepared by electrochemical method(Ag/Qu/WGE).A mixture of L-Thr,chitosan and Nafion was coated on the surface of Ag/Qu/WGE,L-Thr(template molecule) was removed by electrochemical cleaning using potentiostatic method.As a result,a molecular imprinted polymer modified electrode based on chitosan/nano-silver/poly quercetin composites could be obtained for L-Thr detection (TMIP/WGE).The characteristics of the modified electrode were investigated by means of field emission scanning electron microscope technique,X-ray photoelectron spectroscopy,infrared spectra and electrochemical technique.The result indicated that the TMIP/WGE displayed a good electro-catalysis towards the oxidation of L-Thr,which could be used for sensitive and quick detection of L-Thr.The differences of oxidation peak current of L-Thr(1.45 V) showed a good linearity in the concentration range of 0.1-1.0 μ mol/L with a detection limit(3σ) of 0.01 μmol/L.The electrode was successfully applied in the determination of L-Thr in fermentation broth.关键词
L-苏氨酸(L-Thr)/分子印迹膜/纳米银Key words
L-threonine (L-Thr)/molecular imprinted polymer/nano-silver分类
化学化工引用本文复制引用
袁爱国,李斌,陈健平,肖丽杰,晋冠平..L-苏氨酸分子印迹传感器的制备及应用[J].分析测试学报,2017,36(4):519-523,5.基金项目
2014广东省协同创新与平台环境建设专项及公益研究与能力建设专项资金(2014B0901010) （2014B0901010）
