广东药学院学报2017，Vol.33Issue(3)：342-345,4.DOI:10.16809/j.cnki.2096-3653.2016121906
小蓟饮片标准煎液中蒙花苷的TLC鉴别及HPLC含量测定
Identification and determination of linarin in standard decoction of Cirsium setosum by TLC and HPLC
摘要
Abstract
Objective To establish an effective TLC and HPLC method for qualitative and quantitative analysis of standard decoction of Cirsium setosum (Willd.) MB.Methods TLC was applied for quality analysis with acetylacetone-butanone-ethanol-water (1∶3∶3∶13) as the mobile phase and alchlor solution as the chromogenic agent.The content of linarin was determined by HPLC.The separation was carried out on a Kromasil 100-5C18 (250 mm×4.6 mm,5 μm) with methanol-0.5% acetic acid (55∶45) as the mobile phase for isocratic elution at a flow rate of 1.0 mL/min.The detection wavelength was at 326 nm,and the column temperature was at 30 ℃.Besides,the transfer rate of Linarin was also calculated.Results Linarin could be identified by TLC.The characteristic spots in TLC identification were clear and strongly specific.Linarin showed a good liner relationship in a range of 0.11-2.2 μg,r=0.999 9.The average recovery was 99.55%,and RSD was 1.9%.The content of Linarin in 15 batches of standard decoction of C.setosum was among 1.03-1.31 mg/mL,and the transfer rate was 76.34%-88.29%.Conclusion This method is easy and reliable,and it could be used for the quality control of standard decoction of C.setosum.关键词
小蓟/蒙花苷/薄层鉴别/高效液相色谱法Key words
Cirsium setosum(Willd.)MB/linarin/TLC/HPLC分类
中医学引用本文复制引用
刘滢滢,许烈强,黄琼惠,梁远园,谢友良..小蓟饮片标准煎液中蒙花苷的TLC鉴别及HPLC含量测定[J].广东药学院学报,2017,33(3):342-345,4.基金项目
广东省教育部科技部产学研结合项目(2012B090600007) （2012B090600007）
广东省科技计划项目(2013B090600010) （2013B090600010）
