医学分子生物学杂志2017，Vol.14Issue(3)：125-129,5.DOI:10.3870/j.issn.1672-8009.2017.03.001
小鼠髓母细胞瘤干细胞分离培养及干细胞标记物的分析
Mice Medulloblastoma Stem Cells Isolating and Culturing and Its Biomarker Analysis
摘要
Abstract
Objective This study aimed to optimize the method of isolating and culturing tumor stem cells in spontaneous isolating and culturing tumor stem cells in spontaneous medulloblastoma(MB)model mice model mice,and observe the ability of these stem cells to form clone in vitro,and analyze the potential tumor stem cell markers including CD44,CD133 and CD15 through flow cytometry.Methods Tissue of medulloblastoma were dissociated into single cells,which were then cultured in stem cell culture medium.Then we calculated the rate of stem cell sphere formation rate and observed differentiative capacity of stem cells when cultured in serum medium.Finally,the flow cytometry analysis was employed to identify the potential surface marker molecules of stem cells.Results Primary medulloblastoma cells was successfully isolated from the medulloblastoma model mice,which then transform into cell spheroids with advanced self-renewing and proliferating abilities.The cells spheroids could went through the adherence differentiation in serum medium and transform into neuron-like cells.The flow cytometry analysis of stem cells surface markers showed a high expression of CD44 in medulloblastoma,whereas the expressions of CD133 and CD15 are relatively low or showed no variations.Conclusion This study confirms the existence of certain amount of high expression CD44 tumor stem cells in medulloblastoma,which possess the ability to self-renew and proliferate.These tumor stem cells could be isolated and cultured in vitro,grow in iterations,and be induced to differentiate.关键词
髓母细胞瘤/肿瘤干细胞/细胞球/干细胞表面标记/CD44Key words
medulloblastoma/rumor stem cells/sphere/biomarker of stem cells/CD44分类
医药卫生引用本文复制引用
李天骄,张一萌,王庆怡,贾林涛,王婷..小鼠髓母细胞瘤干细胞分离培养及干细胞标记物的分析[J].医学分子生物学杂志,2017,14(3):125-129,5.基金项目
国家自然科学基金(No.81472631) （No.81472631）
This work was supported by a grant from the National Natural Science Foundation of China(No.81472631) （No.81472631）
