广西师范大学学报（自然科学版）2017，Vol.35Issue(2)：108-111,4.DOI:10.16088/j.issn.1001-6600.2017.02.016
超敏化学发光液稀释法降低蛋白印迹的发光强度
Reduced Light Intensity of Western Blot by Diluted Ultra-enhanced Chemiluminescent Solution
摘要
Abstract
Based on specific binding of antigen and antibody,western blot analysis is used to detect a protein in complex samples.However,using conventional chemiluminescence detection,light of the protein band is weak and X film is not much exposed,and using ultra-enhanced chemiluminescence detection,light of the protein band is strong and X film is too much exposed,both development images of protein band are not ideal sometimes,when protein concentration of the samples is too low,especially when the protein to be detected is lower abundant in X film is samples.Therefore,the protein was detected with diluted ultra-enhanced chemiluminescent solution,and the protein band was clearly showed and the development image effect of protein bands was improved obviously,which indicates that the intensity of light emission could be reduced and the development image effect of the protein bands could be improved by dilution of ultra-enhanced chemiluminescent solution in western blot analysis.关键词
蛋白印迹/超敏发光液/化学发光/稀释Key words
western blot/ultra-enhanced chemiluminescent solution/chemiluminescence/dilution分类
医药卫生引用本文复制引用
范才文,唐娟,罗琴,李璐,向秋..超敏化学发光液稀释法降低蛋白印迹的发光强度[J].广西师范大学学报（自然科学版）,2017,35(2):108-111,4.基金项目
国家自然科学基金(81260344) （81260344）
广西自然科学基金(2013GXNSFAA019228) （2013GXNSFAA019228）
广西壮族自治区教育厅资助项目(LX2014274) （LX2014274）
