广西师范大学学报（自然科学版）2017，Vol.35Issue(2)：112-116,5.DOI:10.16088/j.issn.1001-6600.2017.02.017
流式细胞术对生乳中微生物检测的应用研究
The Application of Flow Cytometry in Testing Bacteria of Raw Milk
摘要
Abstract
Flow cytometry (FCM) is a rapid technique for cell-by-cell multiparameter analysis,which is often used in combination with fluorescent labelling for fast viability assessment.FCM analysss with fast and accurate protocol appeared to be workable for food bacteria detection.This article applied enzymatic treatment and membrane filtration method to digest proteins and treatment of milk by centrifugation to remove lipids.Treated milk samples were detected and enumerated by FCM.The bacteria were stained with 7-AAD and counted by fluorescent microspheres.The detection limit of 101CFU/mL was achieved.Raw milk data showed a significant correlation (P<0.01) and a good agreement(r=0.980 2) between FCM and standard plate count methods.关键词
流式细胞术/食品检测/微生物/牛乳Key words
flow cytometry/food detection/bacteria/milk分类
生物科学引用本文复制引用
杨莉婷,何丽,何海宁,吴琼..流式细胞术对生乳中微生物检测的应用研究[J].广西师范大学学报（自然科学版）,2017,35(2):112-116,5.基金项目
国家自然科学基金(31660344) （31660344）
甘肃省动物源性制品安全分析与检测技术重点实验室开放课题(2015-2) （2015-2）
广西大学生创新创业训练项目(201610602052) （201610602052）
