海南医学院学报2017，Vol.23Issue(9)：1262-1264,1268,4.DOI:10.13210/j.cnki.jhmu.20170503.014
肾母细胞瘤过表达基因(NOV)mRNA含量与骨肉瘤组织中细胞生长、迁移相关基因表达的相关性
Correlation of nephroblastoma overexpressed gene (NOV) mRNA levels with cell growth-and migration-related gene expression in osteosarcoma tissue
摘要
Abstract
Objective: To study the correlation of nephroblastoma overexpressed gene (NOV) mRNA levels with cell growth-and migration-related gene expression in osteosarcoma tissue.Methods: 78 patients with osteosarcoma undergoing surgical treatment in our hospital between May 2012 and May 2016 were selected, and the osteosarcoma tissue and tissue adjacent to carcinoma were collected.Fluorescence quantitative PCR method was used to detect the mRNA expression of NOV as well as pro-proliferation/anti-proliferation genes and invasion genes, and the Pearson test was used to further assess the correlation of NOV mRNA expression with the osteosarcoma cell growth and migration activity.Results: NOV mRNA expression in osteosarcoma tissue was significantly lower than that in tissue adjacent to carcinoma, FoxM1, STIM1 and Orai1 mRNA expression were significantly higher than those in tissue adjacent to carcinoma, anti-proliferation genes RanBP9, Tap73, Caspase-3, PTEN and P53 mRNA expression were significantly lower than those in tissue adjacent to carcinoma, and invasion genes EFEMP1, Notch1, Id1 and Src mRNA expression were significantly higher than those in tissue adjacent to carcinoma (P<0.05).Pearson test showed that the NOV mRNA expression in osteosarcoma tissue was negatively correlated with pro-proliferation genes FoxM1, STIM1 and Orai1 mRNA expression, positively correlated with anti-proliferation genes RanBP9, Tap73, Caspase-3, PTEN and P53 mRNA expression, and negatively correlated with invasion genes EFEMP1, Notch1, Id1 and Src mRNA expression (P<0.05).Conclusion: NOV expression is relatively low in osteosarcoma tissue, and NOV mRNA expression is negatively correlated with tumor cell proliferation and invasion activity.关键词
骨肉瘤/肾母细胞瘤过表达基因/增殖基因/侵袭基因Key words
Osteosarcoma/nephroblastoma overexpressed gene/proliferation gene/invasion gene分类
医药卫生引用本文复制引用
梅平均,姜志强,李颖,吕振京..肾母细胞瘤过表达基因(NOV)mRNA含量与骨肉瘤组织中细胞生长、迁移相关基因表达的相关性[J].海南医学院学报,2017,23(9):1262-1264,1268,4.基金项目
佛山市卫生和计生局医学科研课题(2012208) （2012208）
Medical research project of Foshan Bureau of health and family planning (2012208) （2012208）
