湖南中医药大学学报2017，Vol.37Issue(6)：602-605,4.DOI:10.3969/j.issn.1674-070X.2017.06.006
斑马鱼胚胎急性毒性实验法检测赶山鞭化学成分胚胎发育毒副作用
Toxic and Side Effects of Chemical Constituents from Hypericum attenuatum on Embryonic Development of Zebrafish
吴梦华 1董建勇2
作者信息
- 1. 浙江大学医学院附属儿童医院,浙江 杭州 310000
- 2. 温州医科大学,浙江 温州 325000
- 折叠
摘要
Abstract
Objective To detect the toxic and side effects of chemical components from Hypericum attenuatum on embry-onic development of zebrafish by acute toxicity test. Methods The 6 h post-fertilization zebrafish was used as experimen-tal models, randomly divided into the control group with DMSO and the sample groups with quercetin, rutin, hyperin, quercertin -3 -O -α -L -arabino furanoside, quercertin -3 -O -β -D -glucoside of Hypericum attenuatum. The mortality, malformation, hatchability at 72 hpf (72 hours after fertilization) were observed. Results The five constituents show obvious inhibiting hatchability on embryonic development of zebrafish (P<0.05), which show stronger inhibition at high concentra-tion, with dose-dependent effect. Conclusion The five components may have a certain toxicity to embryonic development.关键词
赶山鞭/斑马鱼胚胎/急性毒性Key words
Hypericum attenuatum/embryos of zebrafish/acute toxicity分类
医药卫生引用本文复制引用
吴梦华,董建勇..斑马鱼胚胎急性毒性实验法检测赶山鞭化学成分胚胎发育毒副作用[J].湖南中医药大学学报,2017,37(6):602-605,4.
