湖南中医药大学学报2017，Vol.37Issue(6)：606-610,5.DOI:10.3969/j.issn.1674-070X.2017.06.007
茉莉酸甲酯对茯苓三萜生物合成的调控研究
Study on Regulation of Triterpenoids Biosynthesis in Poria cocos Submerged Fermentation System by Methyl Jasmonate
摘要
Abstract
Objective To explore the regulation effects and its molecular mechanism of methyl jasmonate (MeJA) on triterpenoid biosynthesis in submerged fermentation system of Poria cocos. Methods The effects of addition amount and feeding time of MeJA on Poria cocos triterpenoid biosynthesis were discussed, respectively. Besides, to illustrate regulation mechanism of MeJA, real time PCR (RT-PCR) was employed to analysis the expression level of fps and sqs in mevalonate (MVA) path-way of Poria cocos. Results The optimal regulation strategy was adding 150 μmol/L MeJA on 4th day. Under this condition, the maximum content can reach up to 20.95 mg/L, which was 1.55 times of blank group, 1.32 times of Tween-80 group. RT-PCR results showed that fps and sqs gene expression level both increased significantly, which were 1.17 times, 788.70 times of that in Tween-80 group, respectively. Conclusion MeJA, as an effective exogenous regulatory factor for increasing triter-penoid biosynthesis level of Poria cocos, could efficiently improve triterpenoid biosynthesis level in Poria cocos submerged fer-mentation system.关键词
茯苓/三萜/生物合成/发酵调控/茉莉酸甲酯Key words
Poria cocos/triterpenoids/biosynthesis/regulation of fermentation/methyl jasmonate分类
医药卫生引用本文复制引用
陈林,崔培梧,鲁耀邦,廖彦,张志丽,张书航,黄绍国,易刚强..茉莉酸甲酯对茯苓三萜生物合成的调控研究[J].湖南中医药大学学报,2017,37(6):606-610,5.基金项目
湖南省发改委科研计划(湘发改高技〔2014〕1199号) （湘发改高技〔2014〕1199号）
湖南省中医药科研计划重点项目(201621) （201621）
湖南省教育厅科研计划(16C1219) （16C1219）
湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201404) （ZYFT201404）
湖南中医药大学研究性学习和创新性实验计划(201616) （201616）
湖湘中药资源保护与利用协同创新中心开放基金(00175-12) （00175-12）
湖南中医药大学生物工程重点学科共同资助. （）
