湖南农业大学学报(自然科学版)2017,Vol.43Issue(3):238-243,6.DOI:10.13331/j.cnki.jhau.2017.03.003
江苏水稻主导品种的稻瘟病抗性及抗性基因遗传多样性
Blast resistance of the main rice cultivars in Jiangsu and genetic diversity of blast resistance genes
摘要
Abstract
Forty-nine main rice cultivars of Jiangsu were tested for resistance to rice blasts by inoculating through spraying in seedling stage and by inoculating through injection in booting stage. And 14 molecular markers were used to analyze the genotype and genetic diversity of blast resistance gene. The results showed that no rice cultivar tested exhibited high resistance to leaf blast and neck blast. Among the 49 materials, 5 cultivars exhibited resistance, 7 exhibited moderate resistance, 14 exhibited moderate susceptibility, 12 exhibited susceptibility and 11 exhibited high susceptibility to neck blast; 2 cultivars exhibited resistance, 8 exhibited moderate resistance, 6 exhibited moderate susceptibility, 9 exhibited susceptibility and 24 exhibited high susceptibility to leaf blast. Only 4 cultivars simultaneously exhibited resistance and moderate resistance to leaf blast and neck blast (8.2%). Thirty-five alleles had been tested by 14 molecular markers, variation range of polymorphism information content was 0.0000-0.5375, with an average of 0.3398. Two to eight resistance genes were found for each cultivar, at least five resistance genes were found in 39 materials (79.6 %). The cluster analysis result showed that the 49 cultivars were clustered into 3 groups, and the similarity distance variation range was 0.0000-0.8571, with an average of 0.4039. And 49 rice cultivars dispersed in the clustering figure, indicating frequent exchanges between germplasm resources and high sharing rate of valuable genes.关键词
水稻/主导品种/稻瘟病/抗性鉴定/遗传多样性/江苏Key words
rice/cultivars/rice blast/resistance evaluation/genetic diversity/Jiangsu分类
农业科技引用本文复制引用
李刚,唐久友,储成才,袁彩勇,王健,曹奎荣,李军,孙祥良,程保山,罗伯祥,杨子博,徐卫军..江苏水稻主导品种的稻瘟病抗性及抗性基因遗传多样性[J].湖南农业大学学报(自然科学版),2017,43(3):238-243,6.基金项目
中国科学院战略性先导科技专项(XDA08010401) (XDA08010401)
浙江省科学技术厅项目(2016C32104) (2016C32104)
淮安市应用研究与科技攻关(农业)计划(HAN2015008,HAN201613) (农业)