湖南农业大学学报（自然科学版）2017，Vol.43Issue(3)：274-281,8.DOI:10.13331/j.cnki.jhau.2017.03.010
长沙地区南瓜的小西葫芦黄花叶病毒检测及其分子进化分析
Detection ofZucchini yellow mosaic virus of pumpkin in Changsha and molecular evolution
摘要
Abstract
Zucchini yellow mosaic virus(ZYMV) was detected in 160 suspected pumpkin virus samples in Changsha area, and the detection rate was 46.9%. To further clarify the classification and molecular evolution of ZYMV Changsha isolates, the coat protein gene was cloned and compared with the ZYMV coat protein gene reported in GenBank. And these sequences were analyzed for genetic recombination, phylogenetic, genetic differences, selection pressure, population structure and gene drift analysis. The results showed that no significant recombination of ZYMV coat protein gene was found. Mutation and negative selection are the main driving forces of its evolution, the population structure is relatively stable but is in the trend of expansion. The different isolates of ZYMV coat protein gene form 3 geographically relevant populations: America population, Europe population and Asia population. There is no frequent gene exchange between America and Europe and Asia, and each group may be affected by genetic drift, and gene exchange between Europe and Asia is more frequent.关键词
南瓜/小西葫芦黄花叶病毒/外壳蛋白/分子进化/长沙地区Key words
Zucchini yellow mosaic virus/coat protein/phylogenetic/molecular evolution/Changsha area分类
农业科技引用本文复制引用
刘嘉裕,戴良英,刘勇,张德咏,谭新球,李迅,唐前君..长沙地区南瓜的小西葫芦黄花叶病毒检测及其分子进化分析[J].湖南农业大学学报（自然科学版）,2017,43(3):274-281,8.基金项目
公益性行业(农业)科研专项(201303028) （农业）
国家自然科学基金项目(31101413) （31101413）
湖南省烟草公司项目(13-14ZDAa04,14- 16ZDAa02) （13-14ZDAa04,14- 16ZDAa02）
湖南农业大学神农学者资助基金 （）
湖南省教育厅项目(16K039) （16K039）
