湖南农业大学学报（自然科学版）2017，Vol.43Issue(3)：304-309,6.DOI:10.13331/j.cnki.jhau.2017.03.015
赤眼鳟Toll样受体9基因的cDNA全长克隆及表达特性分析
Molecular cloning and expression characteristics of toll-like receptor gene 9 inSqualiobarbus curriculus
摘要
Abstract
In order to investigate the structural characteristics of the toll-like receptor gene 9 in Squaliobarbus curriculus (ScTLR9), as well as its expression feature on the immune response in case of GCRV infection, the full-length cDNA of gene ScTLR9 was cloned using RACE technology in the study, and the expression ofScTLR9 onimmune response in GCRV infection was analyzed using real-time qPCR. The results showed that the full-length cDNA of ScTLR9 was 3687 bp encoding with a polypeptide of 1059 amino acid residues and containing 17 LRR domains. The ScTLR9 mRNA was expressed in all collected tissues, and the relative expression level in mesonephros and head kidney were higher than that in other tissues; Under GCRV infection, the expression of TLR9 were up-regulated in head kidney for barbel chub and grass carp, the relative expression level reached its peak in 12 h for ScTLR9 in head kidney of barbel chub. It was speculated that the gene ScTLR9 was more quickly on the immune response against GCRV invasion in toll-like receptor than that in grass carp, it played an important role on the response to GCRV infection.关键词
赤眼鳟/Toll样受体9基因/草鱼呼肠弧病毒/草鱼Key words
Squaliobarbus curriculus/toll-like receptor 9(TLR9)/grass carp reovirus (GCRV)/Ctenopharyngodon idella分类
农业科技引用本文复制引用
金生振,王荣华,周智愚,刘巧林,彭慧珍,肖调义..赤眼鳟Toll样受体9基因的cDNA全长克隆及表达特性分析[J].湖南农业大学学报（自然科学版）,2017,43(3):304-309,6.基金项目
国家自然科学基金面上项目(31272652,31572615) （31272652,31572615）
