湖南农业大学学报（自然科学版）2017，Vol.43Issue(3)：315-319,5.DOI:10.13331/j.cnki.jhau.2017.03.017
牛粪堆肥中溶解性有机物对铜绿微囊藻生理生化及产毒特性的影响
Effects of DOM in compost of cattle's manure on the physiological and toxin-producing characteristics ofMicrocystis aeruginosa FACHB905
摘要
Abstract
Dissolved organic matter (DOM) originated from compost of cattle manure had been extracted and added to Microcystis aeruginosa FACHB905 to study its effects on the physiological and toxin-producing characteristics via the determination of cell growth, pigments content and algal toxins content. The concentration of DOM was evaluated by using dissolved organic carbon (DOC). The final concentrations of DOC designed for cultural medium, marked as CK, T1, T2, T3, T4 and T5, were 0, 15, 30, 60, 90 and 120 mg/L, respectively. The results showed that: 1)Cell density influenced by DOM increased in treatments T1 to T5 at day 2; The maximum growth rate occurred in treatment T4 (5.97×106 cells/mL) at day 2, with a 21.67% increase than that of CK. DOM could increase cell growth in treatments T1 to T3 but inhibit cell density in treatments T4 and T5 at day 4, the maximum growth rate occurred in treatment T2 (9.23×106 cells/mL) with a 15.27% increase than that of CK. DOM could only promote the cell growth in treatments T1 and T2, but inhibit cell density in treatments T3 to T5 at day 6, the maximum growth rate occurred in treatment T1 (13.34×106 cells/mL) with a 10.35% increase than that of CK. 2)Compost source of DOM inhibited the synthesis of algal toxins, the lowest content of Microcystin-LR in cells was occurred in treatment T5 at day 2, with a 53.13% decrease than that of CK. The lowest content of Dha7-Microcystin-LR in cells was occurred in treatment T5 at day 4, with a 78.51% decrease than that of CK. 3)There was no significant effect among DOM treatments on the synthesis of Chl-a or carotenoid in cells.关键词
牛粪堆肥/溶解性有机物(DOM)/铜绿微囊藻/藻细胞密度/藻毒素Key words
compost of cattle manure/dissolved organic matter (DOM)/Microcystis aeruginosa FACHB/cell density/microcystins分类
资源环境引用本文复制引用
林毅青,吴根义,许振成,邵继海..牛粪堆肥中溶解性有机物对铜绿微囊藻生理生化及产毒特性的影响[J].湖南农业大学学报（自然科学版）,2017,43(3):315-319,5.基金项目
科技部水专项(2014ZX07206001-03) （2014ZX07206001-03）
