解放军医学杂志2017，Vol.42Issue(1)：17-22,6.DOI:10.11855/j.issn.0577-7402.2017.01.04
活性氧在热打击诱导肠上皮细胞凋亡中的作用
Role of reactive oxygen species in apoptosis of intestinal endothelial cells induced by heat stress
摘要
Abstract
Objective To observe the oxidative stress, integrity of lysosome and apoptosis of intestinal epithelial cells 6 (IEC-6) after heat stress, and explore the pathogenesis of intestinal damage caused by heat stress.Methods In the heat stress groups,the cells were incubated at 43℃ for 1 hour, then, further incubated at 37℃ and 5% CO2 for 0, 1, 3, 6 and 12 hours respectively; in the medicine intervention group, the cells were pretreated with the medicine 1h before heat stress; while in control group, the cells were incubated at 37℃ and 5% CO2. The amount of reactive oxygen species (ROS) was assayed with 2', 7'-dichlorofluorescin diacetate (DCFH-DA) and dihydroethidium (DHE) staining. The stability of lysosome membrane was checked by AO staining. Apoptosis was analyzed by flow cytometry using annexinⅤ-FITC/PI staining, CCK-8 assay was used to assess cellular viability.Results Compared with control group, cell viability decreased and apoptosis increased at 1 h after heat stress, which was the most obvious at 12h after rewarming (P<0.05). While ROS and pale cells increased immediately after heat stress and the increase become the most obvious (P<0.05). The cell viability in E-64 pretreatment group was significantly improved such as apoptosis reduction, compared with heat stress group (P<0.05).Conclusion Heat stress could induce robust increase of ROS, which mediates lysosome damage and results in cell apoptosis, thus suggesting that ROS-lysosome pathway may play an important role in intestinal injury in heat stress.关键词
热打击/细胞凋亡/活性氧/肠上皮细胞Key words
heat stress/apoptosis/reactive oxygen species/intestine epithelial cells分类
医药卫生引用本文复制引用
何旋,古正涛,李莉,邹志敏,潘志国,苏磊..活性氧在热打击诱导肠上皮细胞凋亡中的作用[J].解放军医学杂志,2017,42(1):17-22,6.基金项目
广东省自然科学基金研究团队项目(S2013030013217) （S2013030013217）
广东省自然科学基金面上项目(2014A030313601) （2014A030313601）
广东省科技计划项目(2013B031800010)This work was supported by the Guangdong Provincial Natural Science Fund Subject (S2013030013217), the General Program of Natural Science Foundation of Guangdong Province (2014A030313601), and the Science and Technology Project of Guangdong Province (2013B031800010) （2013B031800010）
