解放军医学杂志2017，Vol.42Issue(1)：34-40,7.DOI:10.11855/j.issn.0577-7402.2017.01.07
FUT2和FUT3基因多态性及其表达与溃疡性结肠炎的关系
Association of ulcerative colitis with FUT2 and FUT3 polymorphisms and their expression in Chinese patients
摘要
Abstract
Objective To explore the association of genetic polymorphism of fucosyltransferase (FUT) 2 and FUT3 and expression of Lewis antigen with ulcerative colitis (UC) in Chinese Zhejiang Han population.Methods We genotyped FUT2 (rs281377, rs1047781 and rs601338) and FUT3 (rs28362459, rs3745635 and rs3894326) in 485 UC patients and 580 healthy controls using SNaPshot. By immunohistochemistry method, we also evaluated expression of Lewis a and b antigens in the sigmoid colon of 10 UC patients and 10 patients with benign colonic polyps.Results The frequencies of mutant allele (A) and genotype (GA+AA) in FUT3 rs3745635 were higher in UC patients than in controls (P=0.016, 95%CI 1.339-1.699;P=0.038, 95%CI 1.330-1.742, respectively). Stratified analyses revealed that the frequencies of mutant allele (G) and genotype (TG+GG) of FUT3 rs28362459 were significantly lower in patients with extensive colitis than in those with distal colitis (P=0.001, 95%CI 0.567-0.786;P<0.001, 95%CI 0.503-0.742, respectively). Similar conclusions were drawn for the mutant allele (A) and genotype (GA+AA) of FUT3 rs3745635 in patients with extensive colitis compared to those with distal colitis (P=0.011, 95%CI 0.621-0.900;P=0.006, 95%CI 0.553-0.845, respectively). Although expression of Lewis b antigen in the sigmoid colon did not differ between UC patients and controls, Lewis a antigen expression was higher in the crypt epithelium of both inflammatory and non-inflammatory sigmoid colon of UC patients than in controls (P=0.028).Conclusion Polymorphisms of FUT3 and expression level of Lewis a antigen might be associated with UC.关键词
结肠炎,溃疡性/岩藻糖基转移酶/血型抗原Key words
colitis/ulcerative/fucosyltransferase/blood group antigens分类
医药卫生引用本文复制引用
吴昊,叶方鹏,王文星,林秀清,林道泼,金捷,蒋益..FUT2和FUT3基因多态性及其表达与溃疡性结肠炎的关系[J].解放军医学杂志,2017,42(1):34-40,7.基金项目
浙江省自然科学基金(LY14H030012,LY15H030018),浙江省卫生厅资助(2012KYA132),温州市科技局资助项目(Y20140533,Y20150157,Y20150029)This work was supported by the Natural Science Funding of Zhejiang Province (LY14H030012, LY15H030018) （LY14H030012,LY15H030018）
Health Department of Zhejiang (2012KYA132) （2012KYA132）
and the Science and Technology Bureau of Wenzhou (Y20140533, Y20150157, Y20150029) （Y20140533, Y20150157, Y20150029）
