解放军医学杂志2017，Vol.42Issue(5)：372-376,5.DOI:10.11855/j.issn.0577-7402.2017.05.03
人脐带间充质干细胞对大鼠重症急性胰腺炎的保护作用
Protective effect of human umbilical cord-derived mesenchymal stem cells against severe acute pancreatitis in rats
摘要
Abstract
Objective To study the protective effects of human umbilical cord-derived mesenchymal stem cells (ucMSCs)against severe acute pancreatitis (SAP) in rats.Methods A total of 135 Sprague-Dawley male rats were randomly divided into Sham group,SAP group and SAP+ucMSCs group (45 each).SAP+ucMSCs group:Severe acute pancreatitis was induced by injecting 5％ sodium taurocholate (0.1ml/100g) into the common bilio-pancreatic duct and then CM-DiI-labeled ucMSCs at 1 × 107cells/kg were injected via the tail vein.All the rats were sacrificed 12,24 and 72 hours after SAP.The 72h death rate was counted.Pathological changes in the pancrease were detected by HE staining and pathological score was graded,ucMSCs colonization was observed by fluorescence microscopy.The serum levels of amylase,lipase,TNF-α,IL-1β,IL-4 and IL-10 were determined by ELISA.Results ucMSCs colonize the injured area of pancreatic tissue,the 72h death rate was reduced,and the serum amylase and lipase were also reduced significantly.Moreover,ucMSCs significantly reduced the pathological score of the pancrea and the level of proinflammatory cytokines (TNF-α and IL-1β),but the levels of anti-inflammatory cytokines were increased (IL-4 and IL-10).Conclusion Transplantation of ucMSCs can reduce the severity of pancreatic injury and inflammation in SAP rats.关键词
间质干细胞/胰腺炎,急性坏死性/炎症Key words
mesenchymal stem cells/pancreatitis,acute necrotizing/inflammation分类
医药卫生引用本文复制引用
吴东叶,孙红玉,杨冠,肖和达,刘卫辉,梁鸿寅,杨莉,汤礼军..人脐带间充质干细胞对大鼠重症急性胰腺炎的保护作用[J].解放军医学杂志,2017,42(5):372-376,5.基金项目
国家自然科学基金(81500409)This work was supported by the National Natural Science Foundation (81500409) （81500409）
