解放军医学杂志2017，Vol.42Issue(5)：377-382,6.DOI:10.11855/j.issn.0577-7402.2017.05.04
骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用
Role of bone marrow mesenchymal stem cells in repair of neural cell injury induced by okadaic acid
摘要
Abstract
Objective To study whether the human bone marrow mesenchymal stem cells (HBMSCs) can repair damaged neural cells induced by okadaic acid (OA).Methods Neuroblastoma cell line SH-SY5Y cells were used to incubate with 20nmol/L okadaic acid for 24h,establishing Alzheimer's Disease cell model;Three groups were set up:normal group,okadaic acid-damaged (OA-damaged) group,hBMSCs-treatment group.The cells were injured for 24h with 20nmol/L OA in OA-damaged group,and treated with conditioned medium obtaining hBMSCs for 24h after 24h OA injury in the treatment group.Then CCK-8 was used for detecting cell vitality,immune fluorescence dyed microtubules and micro filaments for determining the dendritic cell length and fluorescence intensity,in addition,Western blotting for analyzing the protein level of phosphorylated tau and total tau proteins.Results Okadaic acid damaged SH-SY5Y cells,contributed to shrinkage,collapse,cavitation of the SH-SY5Y cell body,dendritic shortening and fracture,and irregular arrangement of microtubule microfilaments;while BMSCs conditioned medium made SHSYSY cell body become round and longer,dendrites restored,and microtubules and microfilaments arranged regularly,fluorescence intensity enhanced.Meanwhile,it also down-regulated the level of OA-induced tau phosphorylation.Conclusion hBMSCs have repair effects on the neural cell damage induced by okadaic acid.关键词
骨髓间充质干细胞/冈田酸/tau蛋白质类/阿尔茨海默病Key words
bone mesenchymal stem cells/okadaic acid/tau proteins/Alzheimer disease分类
医药卫生引用本文复制引用
刘嘉婧,曹宁,翟晶磊,廖土玲,岳文,贾雅丽,裴雪涛..骨髓间充质干细胞对冈田酸致神经细胞损伤的修复作用[J].解放军医学杂志,2017,42(5):377-382,6.基金项目
国家高技术研究发展计划(863计划)项目(2013AA020107) （863计划）
广州市健康医疗协同创新重大专项(201604020009) （201604020009）
广东省重大科技专项(2013A022100005) （2013A022100005）
广东省前沿与关键技术创新专项(2014B020228001,2015B020226001)This work was supported by the National High Technology Research and Development Program (863 Program) of China (2013AA020107),Guangzhou Health Care and Cooperative Innovation Major Project (201604020009),Guangdong Major Scientific and Technological Project (2013A022100005),and Guangdong Frontier and Key Technology Innovation Project (2014B020228001,2015B020226001) （2014B020228001,2015B020226001）
