解放军医学杂志2017，Vol.42Issue(5)：402-406,5.DOI:10.11855/j.issn.0577-7402.2017.05.08
应用基质辅助激光解吸电离飞行时间质谱研究细菌性血流感染的血清多肽指纹图谱
Studies on the serum peptide fingerprint of bacterial bloodstream infection by matrix-assisted laser desorption ionization time of flight mass spectrometry
麻雅婷 1杨明 2何赏 2陈琛 2张可昕 2王成彬2
作者信息
- 1. 325035 浙江温州 温州医科大学检验医学院生命科学院
- 2. 100853 北京 解放军总医院临床检验科
- 折叠
摘要
Abstract
Objective To study the serum peptide fingerprint using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technology,and find the different peaks with potential significance and establish the diagnosis model of Staphylococcus aureus and Escherichia coli bloodstream infection.Methods To establish ICR mice model of S.aureus and E.coli bloodstream infections,and collect serum samples.The serum samples were purified by weak cation exchange beads,the serum peptide fingerprint was recognized by using MALDI-TOF MS and BioExplorer software between infections group and normal control group.Results Compared with the normal control group,6 peptides were up-regulated,7 peptides downregulated and 8 peptides up-regulated first and then down-regulated in S.aureus infection group;And 5 peptides down-regulated,4 peptides down-regulated first and then up-regulated,and 8 peptides up-regulated first and then down-regulated in E.coli infection group.Conclusion MALDI-TOF MS combined with BioExplorer software may be used as a tool to study the serum peptides of S.aureus and E.coli bloodstream infection,effectively find significant peptides for establishing a diagnosis model of these two bacterial infections,and has a certain value for the diagnosis of bacterial bloodstream infection.关键词
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)/细菌性血流感染/大肠埃希菌/金黄色葡萄球菌/血清多肽指纹图谱Key words
matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS)/bacterial bloodstream infection/Escherichia coli/Staphylococcus aureus/serum peptide profiling分类
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麻雅婷,杨明,何赏,陈琛,张可昕,王成彬..应用基质辅助激光解吸电离飞行时间质谱研究细菌性血流感染的血清多肽指纹图谱[J].解放军医学杂志,2017,42(5):402-406,5.
