解放军医学杂志2017，Vol.42Issue(6)：495-499,5.DOI:10.11855/j.issn.0577-7402.2017.06.03
抗氧化剂对热打击人脐动脉血管平滑肌细胞收缩反应的影响
Influence of antioxidants on the contractile response of heat-stressed human umbilical artery smooth muscle cells
摘要
Abstract
Objective To study the change of the contractile response of human umbilical artery smooth muscle cells (HUASMCs) during the heat stress, and explore the effect of the antioxidant on the changes. Methods HUASMCs were randomly divided into control group, heat stress group, antioxidant preprocessing group. Cells were stimulated by norepinephrine (NE) at a low concentration (0.05mg/L) and at a normal concentration (1.0mg/L) and cultured in the thermostatic water bath (41℃) for 0.5, 1, 1.5 or 2h, respectively. After stimulated by NE, proportion of the cell surface area contraction was measured to reflect the contractile response of each group. Results Compared with control group, regardless of the NE concentration: in heat stress group, contractile response at 1h increased significantly (P<0.05), while at 2h, it was reduced significantly (P<0.05 or 0.01). In the antioxidant preprocessing group, the contractile response was reduced significantly from heat stress to 2h after heat stress (P<0.05 or 0.01). There was no statistically significant difference in contractile response between different NE concentrations in the control group and heat stress group (P>0.05), but in the antioxidant preprocessing group, the contractile response was more significant to the normal NE concentration than to the low NE concentration (P<0.05 or 0.01). Regardless of the NE concentration, the contractile response was lower in the antioxidant preprocessing group than in the heat stress group. Conclusions In the course of heat stress, the contractile response of HUASMCs presents as time-related change. The usage of antioxidant may correct the over-response of HUASMCs to NE in the early heat stress stage, but cannot correct the reduction of the contractile response in the middle and advanced stage.关键词
热打击/肌细胞,平滑肌/抗氧化剂Key words
heat stress/myocytes/smooth muscle/antioxidants分类
医药卫生引用本文复制引用
李志鹏,金慧,童华生,刘志锋,徐秋林,苏磊..抗氧化剂对热打击人脐动脉血管平滑肌细胞收缩反应的影响[J].解放军医学杂志,2017,42(6):495-499,5.基金项目
国家自然科学基金(81471839,81671896)This work was supported by the National Natural Science Foundation of China (81471839, 81671896) （81471839,81671896）
