解放军医学杂志2017，Vol.42Issue(6)：506-510,5.DOI:10.11855/j.issn.0577-7402.2017.06.05
谷氨酰胺对热打击下单层肠上皮Caco-2细胞屏障功能的影响
Influence of glutamine on barrier function of heat-stressed intestinal epithelial Caco-2 cells
摘要
Abstract
Objective To investigate the effect of Glutamine (Gln) on heat stress-induced dysfunction of intestinal epithelial barrier. Methods Human intestinal epithelial Caco-2 cells were pre-incubated with Gln for 24h and then exposed to heat 43℃ for 1h. Cell counting kit-8 (CCK-8) was used to detect the cellular proliferation with various concentrations of Gln and choose an optimum concentration for subsequent experiments. The barrier integrity was measured by transepithelial electrical resistance (TEER) and horseradish peroxidase (HRP) permeability. Levels of tight junction protein occludin and ZO-1 were analyzed by Western blotting. Cytoskeleton using Coomassie blue staining was observed by microscopy. Results At 0.7mmol/L concentration, Gln showed the most effective cell proliferation compared with other concentration groups (P<0.05). Therefore, 0.7mmol/L Gln was used as effective concentration in following experiments. Gln attenuated the TEER decrease and impairment of intestinal permeability induced by heat exposure compared with 43℃ group (P<0.01). The expressions of occludin and ZO-1 were significantly elevated by pretreatment with Gln. The distortion of cytoskeleton was also effectively prevented. Conclusion 0.7mmol/L Gln is potentially beneficial for protecting against heat stress-induced permeability dysfunction and epithelial barrier damage.关键词
热打击/谷氨酰胺/肠黏膜Key words
heat stress/glutamine/intestinal mucosa分类
医药卫生引用本文复制引用
肖桂珍,李俊,易万华,罗家劲,苏磊..谷氨酰胺对热打击下单层肠上皮Caco-2细胞屏障功能的影响[J].解放军医学杂志,2017,42(6):506-510,5.基金项目
广东省自然科学基金(S2013030013217)This work was supported by the Natural Science Foundation of Guangdong Province (S2013030013217) （S2013030013217）
