实用临床医药杂志2017，Vol.21Issue(9)：94-98,102,6.DOI:10.7619/jcmp.201709024
胶质细胞源性神经营养因子修饰的骨髓间充质干细胞移植治疗脊髓损伤的效果
Effect of bone marrow mesenchymal stem cells modified by GDNF in transplantation treatment of patients with spinal cord injury
摘要
Abstract
Objective To observe the effectiveness of transplantation of neuron-like cells induced by GDNF from BMSCs on treatment of spinal cord injury in rats.Methods Transfected BMSCs by recombinant adenovirus containing the gene of rGDNF and GFP.36 SD rats with spinal cord injury were randomly divided into three groups.All the 36 SD rats with spinal cord injury were scored by the Basso,Beattle,Bresnahan locomotoRrating scale at different time points (1、2、3、4 weeks afteRoperation).In the 4th week afteRoperation,tissue from the injury section was prepared to observe repaiRof spinal cord injury.Results BMSCs was able to secrete GDNF continuously afteRtransfected by recombinant adenovirus containing the gene of rGDNF and GFP.AfteRoperation,BBB scores in intralesional treatment group significantly increased when compared with that of intravenous treatment group and injury control group.In the 4th week afteRoperation,HEmatoxylin-eosin staing results showed that cavitation in intravenous treatment group was largeRthan that in intralesional treatment group with the one of injury control group being the biggest.Immunohistochemical method showed that there were more NF200+cells and GFAP+cells in intralesional treatment group than in the otheRtwo groups,and injury control group had the least NF200+cells and GFAP+ cells.Conclusion AfteRinduced into nuron-like cells,BMSCs can treat spinal cord injury effectively.关键词
脊髓损伤/骨髓间充质干细胞/胶质细胞源性神经营养因子Key words
spinal cord injury/bone marrow mesenchymal stem cells/Gial cell line-derived neurotrophic factor分类
医药卫生引用本文复制引用
吴朗,黄成,冯新民,毕松超,陈涛,王鹏,杨建东..胶质细胞源性神经营养因子修饰的骨髓间充质干细胞移植治疗脊髓损伤的效果[J].实用临床医药杂志,2017,21(9):94-98,102,6.基金项目
国家自然科学基金面上项目资助项目(81071466) （81071466）
江苏省六大人才高峰资助项目(2014-WSN-076) （2014-WSN-076）
