实用临床医药杂志2017，Vol.21Issue(9)：119-122,4.DOI:10.7619/jcmp.201709031
脓毒症患儿CD14+单核细胞中miR-146a的表达及其临床意义
Expression and clinical significance of miRNA-146a in CD14+ MC of children with sepsis
摘要
Abstract
Objective To investigate the expression of miR-146a in CD14+ monocytes (MC) of children with sepsis.Methods Forty children with sepsis in PICU were enrolled in the study.Fifteen children with systemic inflammatory response syndrome (SIRS) afteRsurgical treatment and fifteen healthy children were selected as control group.Expressions of miR-146a and TNF-α,IL-10 of CD14+ MC were detected by real-time quantitative PCR(qRT-PCR).HLA-DRlevels of CD14+ MC were detected by flow cytometry.Levels of high-sensitivity C-reactive protein (CRP) and procalcitonin (PCT) were detected.Results The HLA-DRlevels of CD14+ MC in all the children with sepsis were oveR30%.The miR-146a level of CD14+ MC were significantly higheRin sepsis group than SIRS group and healthy controls (P<0.05),and the level decreased when sepsis children were recovered (P<0.05).The TNF-α,IL-10 level of CD14+ MC in children with sepsis was significantly higheRthan healthy controls (P<0.05).Conclusion The expressions of CD14+ MC miR-146a in children with sepsis are significantly up-regulated.关键词
儿童/微小RNA-146a/脓毒症/CD14+单核细胞Key words
children/miR-146a/sepsis/CD14+ monocytes分类
医药卫生引用本文复制引用
武宇辉,齐颖,马伟科,李雨铮,杨卫国,李成荣..脓毒症患儿CD14+单核细胞中miR-146a的表达及其临床意义[J].实用临床医药杂志,2017,21(9):119-122,4.基金项目
广东省深圳市科技计划项目(JCYJ20140416141331523) （JCYJ20140416141331523）
