军事医学2017，Vol.41Issue(5)：338-341,4.DOI:10.7644/j.issn.1674-9960.2017.05.003
芋螺毒素GI抗血清制备及中和活性研究
Preparation of conotoxin GI antiserum and determination of neutralization activity
摘要
Abstract
Objective To determine whether the antiserum produced by immunizing mice with conotoxin GI coupled with bovine serum albumin (BSA) could neutralize GI conotoxin.Methods The GI-BSA was prepared by glutaraldehyde-coupled method,and the mice were immunized with the GI-BSA to produce antiserum.The antibody neutralization assay was used to test the detoxication of the antiserum.Results The SDS-PAGE protein electrophoresis showed that the coupling reaction of GI hapten with BSA was successful.The two distinct protein bands of GI-BSA were more than 120×103.Each mouse was immunized four times with 99 μg every two weeks.After the fourth immunization,the serum neutralization titer was more than 1:64 000.After the intraperitoneal injection of the mixture of 100 or 200 μl of the antiserum and different doses of GI,75% of the mice survived in the group with 100 μl of the antiserum and 1× LD50 GI(16.3 μg/kg).The same percentage of mice also survived in the group of with 200 μl of serum and 25.8 μg/kg of GI.Conclusion The antiserum produced by immunizing mice with GI-BSA exhibits significant detoxication activity to conotoxin GI.关键词
芋螺毒素类/牛血清白蛋白/偶联/免疫/中和活性Key words
conotoxins/bovine serum albumin/coupling/immunization/neutralization activity分类
医药卫生引用本文复制引用
唐艺菲,胡小波,余硕,戴秋云..芋螺毒素GI抗血清制备及中和活性研究[J].军事医学,2017,41(5):338-341,4.基金项目
国家重点研发计划资助项目(2016YFC1202902) （2016YFC1202902）
