军事医学2017，Vol.41Issue(5)：377-380,389,5.DOI:10.7644/j.issn.1674-9960.2017.05.011
尾吊对空间诱变肺炎克雷伯菌感染小鼠炎症反应的增强作用
Increased inflammatory reaction in tail-suspension mice infected by K.pneumoniae from spaceflight
摘要
Abstract
Objective To explore the changes in inflammatory reactions in tail-suspension mice infected by Klebsiella pneumoniae from spaceflight.Methods Tail suspension was used to simulate the physiological effects of microgravity.C57BL/6 mice were randomly divided into control (Con),control+K.pneumoniae T16-169 (Con+T16-169),tail suspension (TS) and tail suspension+K.pneumoniae T16-169 (TS+T16-169) groups.The level of inflammatory cytokines TNF-α,IL-6 and IL-1β mRNA in lung tissue and the plasma cytokine concentration were detected by RT-qPCR and xMAP technology,and HE staining was used to represent the morphological changes in lung tissue.Results Compared with the control group,the expression of inflammatory cytokines in lung tissue and plasma concentrations of all experimental groups were increased,and the difference in TS+T16-169 group was the most significant (P<0.01 or P<0.001).HE staining showed that the lung tissues in Con+T16-169 and TS+T16-169 groups were damaged in different degrees,and the damage of TS+T16-169 group was the most serious.Conclusion The K.pneumoniae from spaceflight significantly increases the expression of inflammatory cytokines in lung tissue and plasma concentrations after infecting tail-suspension mice,and induces more serious damages to the lung tissue,which suggests that inflammatory reactions can be increased in tail suspension mice infected by K.pneumoniae from spaceflight.关键词
空间诱变/肺炎克雷伯菌/模拟失重/炎症因子/肺组织Key words
space mutation/Klebsiella pneumoniae/simulated microgravity/inflammatory cytokines/lung tissue分类
医药卫生引用本文复制引用
刘蓉,程江,裴雪枫,贾茗雯,王静宇,王俊锋,刘长庭,袁明..尾吊对空间诱变肺炎克雷伯菌感染小鼠炎症反应的增强作用[J].军事医学,2017,41(5):377-380,389,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(2014CB744404) （973计划）
