河南科技大学学报（医学版）2017，Vol.35Issue(2)：85-87,91,4.DOI:10.15926/j.cnki.issn1672-688x.2017.02.002
过表达BCL-2对急性脑梗死大鼠神经细胞凋亡及相关基因的影响
Effects of BCL-2 Overexpression on Neuronal Apoptosis and Related Genes in Rats with Acute Cerebral Infarction
摘要
Abstract
Objective To investigate the effects of BCL-2 overexpression mediated bylentivirus on neuronal apoptosis and related genes in rats with acute cerebral infarction.Methods 45 rats were randomly divided into sham group (only isolated vascular suture unplugged), model group and observation group.At 10 days before operation, rats in sham group and model group were treated with empty vector mediated by lentiviral;rats in observation group were treated with BCL-2 overexpression mediated by lentivirus null carrier.After middle cerebral artery infarction model were constructed at 72 h, the caspase-3, BCL-2, Bax protein apoptosis index of rat brain were analyzed.Results BCL-2 protein mediated by lentivirus could be expressed in rat brain.BCL-2 protein levels in observation group was significantly higher than that of sham group and model group(all P<0.05).Caspase-3 and Bax protein levels in model group was significantly higher than that of sham group(all P<0.05), while caspase-3 and Bax protein levels in observation group were significantly lower than that of model group(all P<0.05) , but it were significantly higher than that of sham group(all P<0.05).Apoptosis in model group and observation group was significantly higher than that of sham group (all P<0.05).Conclusion Overexpression of BCL-2 could reduce the level of apoptosis in rat of acute cerebral infarction and have a protective effect on brain.关键词
脑梗死/BCL-2/细胞凋亡/caspase-3/Bax/大鼠Key words
cerebral infarction/BCL-2/apoptosis/caspase-3/Bax/rat分类
医药卫生引用本文复制引用
彭静华,张育德,张鸿日,闫俊强..过表达BCL-2对急性脑梗死大鼠神经细胞凋亡及相关基因的影响[J].河南科技大学学报（医学版）,2017,35(2):85-87,91,4.基金项目
1.国家自然科学基金(U1304809) （U1304809）
2.河南洛阳市青年医学科技创新联合专项资金项目(1503008A-15) （1503008A-15）
