林业科学2017，Vol.53Issue(5)：107-115,9.DOI:10.11707/j.1001-7488.20170513
马缨丹提取物对黄胸散白蚁体内酶活性的影响
Effects of Extracts of Lantana camara (Verbenaceae) Leaves on Cellulose and Detoxifying Enzymes Activities of Reticulitermes flaviceps (Isoptera: Rhinotermitidae)
摘要
Abstract
[Objective] In order to explore the action mechanisms,the effects of 3 different solvent extracts from Lantana camara (Verbenaceae) leaves on cellulose and detoxification enzyme in the termite Reticulitermes flaviceps (Isoptera: Rhinotermitidae) were studied.[Method] Termites were fed with filter paper saturated with 3 different solvent extracts of L.camara leaves for different times,and then we measured the enzymes activities of endo-β-1,4-glycanase (EG),exo-β-1,4-glucanase (CBH),β-1,4-glucosidase (BG),carboxylesterase (CarE),glutathione-S-transferase (GSTs) and mixed-functional oxidase (MFO).[Result] The all extracts had to some degree inhibiting effects on the six enzymes activities,however there were differences in the inhibition with extracts of chloroform,ethyl acetate and petroleum ether at 6-72 h.As for the three cellulose enzymes,the chloroform extract had no significant effect on EG,but had significantly inhibiting effect on CBH and BG,especially on CBH with the highest inhibition rate of 17.61% at 72 h.The ethyl acetate extract also had inhibiting effect on the 3 enzymes activities,with relatively stronger inhibition on EG and BG,and the inhibition rate on EG was 27.18% at 48 h,and 29.28% at 24 h,respectively.The petroleum ether extract had no significant effect on CBH,but had strongly inhibited on EG and BG,with the highest inhibition rate of 39.89% on EG at 72 h.As for the three detoxification enzymes,the 3 extracts had no significant effect on CarE,but had inhibiting effect on GSTs,which mainly occurred at 12-48 h.The ethyl acetate extract had the strongest inhibition,with the highest inhibition rate of 39.54% at 36 h.The MFO activities fluctuated after being treated with 3 extracts,and the relatively stronger inhibition on the 3 detoxification enzymes occurred at 12 h and 36 h.[Conclusion] The extracts of chloroform,ethyl acetate and petroleum ether could decrease the cellulose and detoxification enzymes activities,disrupting the normal physiological metabolism.关键词
黄胸散白蚁/马缨丹提取物/纤维素酶/解毒酶/杀虫作用机制Key words
Reticulitermes flaviceps/Lantana camara extract/cellulose enzyme/detoxification enzyme/insecticidal mechanism分类
农业科技引用本文复制引用
孙骊珠,罗兰,袁忠林..马缨丹提取物对黄胸散白蚁体内酶活性的影响[J].林业科学,2017,53(5):107-115,9.基金项目
国家自然科学基金项目(31272106) （31272106）
山东省"泰山学者"建设工程专项. （）
