生态与农村环境学报2017，Vol.33Issue(6)：571-576,6.DOI:10.11934/j.issn.1673-4831.2017.06.012
A2/O工艺处理污水中雌激素活性和典型雌激素水平变化
Activity of Estrogen and Contents of Typical Estrogenic Compounds in A2/O Treated Wastewater
摘要
Abstract
As endocrine disrupting chemicals in the water environment is low in concentration, difficult to remove and highly hazardous, it is essential to get a full grasp of how estrogen in wastewater varies with the process of treatment in activity and concentration.To that end, samples of wastewater were collected from an urban wastewater treatment installation for analysis of concentrations and activities of 4 kinds of steroid estrogens, using the high performance liquid chromatography-UV detector-fluorescence detector (HPLC-UV-FLD) and the yeast estrogen screen (YES) method, separately.Moreover, enzyme linked immunosorbent assay (ELISA) was performed to determine concentrations of E2 in the wastewater under treatment.Results show that among the four kinds of estrogens detected in the effluent, E1 was the highest in concentration, reaching 8797 ng·L-1 and E2, the lowest being 550 ng·L-1, which indicates that the steroid estrogen removal rate varied in the range of 8883%-9569%.The A2/O system was found to be able to lower activity of the estrogens by 7333%.However, the estrogens in the effluent were still quite active with EEQ being 192 ng·L-1.ELISA reveals that the concentration of E2 in the effluent varied in the range of 1001-8282 ng·L-1, which indicates that the removal rate of E2 was 8823%.Obviously ELISA and YES are quite consistent in determination.Therefore, it could be concluded that ELISA could be a workable supplement to in vitro assays of estrogens.关键词
A2/O工艺/雌激素/雌激素活性/酶联免疫吸附法(ELISA)/去除率Key words
A2/O process/estrogens/estrogenicity/ELISA/removal rate分类
资源环境引用本文复制引用
游猛,张秋亚,王晓昌,马晓妍..A2/O工艺处理污水中雌激素活性和典型雌激素水平变化[J].生态与农村环境学报,2017,33(6):571-576,6.基金项目
城区水污染控制和水环境功能提升技术研究项目(2014ZX07305-002-01) （2014ZX07305-002-01）
国家自然科学基金(51508449) （51508449）
中国博士后科学基金面上项目(2015M572531) （2015M572531）
