南京工业大学学报（自然科学版）2017，Vol.39Issue(3)：52-57,6.DOI:10.3969/j.issn.1671-7627.2017.03.010
基于大肠杆菌MG1655载体矿化合成纳米硒的研究
Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary
摘要
Abstract
The reduction with Escherichia coli MG1655 of sodium selenite was used to prepare nano-selenium with different mass concentrations of sodium selenite as selenium source artificially.The influence of sodium selenite mass concentration on the growth curve of Escherichia coli MG1655 was investigated.The reduction efficiency of sodium selenite was determined by hydride generation atomic fluorescence spectrometry (HG-AFS),and the structure of the product was characterized by scanning electron microscope (SEM),X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR).Results indicated that sodium selenite could be biomineralized in abundance by Escherichia coli MG1655 to nano-selenium particles in red simple substance with their diameters mainly 100~250 nm.Moreover,all the reduction rates of various mass concentrations of sodium selenite solution were over 50%.关键词
大肠杆菌MG1655/Na2SeO3/纳米硒/表征Key words
Escherichia coli MG1655/sodium selenite/nano-selenium/characterization分类
生物科学引用本文复制引用
武童,汪振炯,华春,周峰,王仁雷,黄和..基于大肠杆菌MG1655载体矿化合成纳米硒的研究[J].南京工业大学学报（自然科学版）,2017,39(3):52-57,6.基金项目
国家自然科学基金(21376112) （21376112）
江苏省自然科学基金(BK20131344) （BK20131344）
