南京工业大学学报（自然科学版）2017，Vol.39Issue(3)：58-62,89,6.DOI:10.3969/j.issn.1671-7627.2017.03.011
一株石油烃高效降解菌的筛选及降解性能研究
Screening of a high efficient bacterial strain for petroleum hydrocarbons degradation and its characteristics
董丁 1卢彦珍 1唐美华 1陈国松1
作者信息
- 1. 南京工业大学 化学与分子工程学院,江苏南京211800
- 折叠
摘要
Abstract
Domestication by using base oil-inorganic salt culture medium,separation with Luria-Bertani (LB) plate and oil stain analysis,and a bacterial strain for petroleum hydrocarbons degradation was separated and screened from oily sludge.Morphologic observation and 16Sr RNA gene sequence identification indicated that it belonged to Bacillus subtilis with high ability to degrade petroleum hydrocarbons.Treating 5 g oily sludge by using 10 mL bacteria liquid,the petroleum hydrocarbons degradation rate achieved 33.52% and 90.61% separately after 7 d and 90 d.If nutrient solution was regularly supplied,the degradation rate would increased to 90.98% after 60 d with an obviously shortened time.The oil content of the sludge was 0.28%,lower than the limiting value 0.3% in the national standard of agricultural soil.This bacterial strain showed a good application prospect for the treatment of oily sludge and remediation of soil contaminated by crude oil.关键词
石油烃/降解/枯草芽孢杆菌/含油泥砂Key words
petroleum hydrocarbon/degradation/Bacillus subtilis/oily sludge分类
能源科技引用本文复制引用
董丁,卢彦珍,唐美华,陈国松..一株石油烃高效降解菌的筛选及降解性能研究[J].南京工业大学学报（自然科学版）,2017,39(3):58-62,89,6.
