乳业科学与技术2017，Vol.40Issue(2)：27-29,3.DOI:10.15922/j.cnki.jdst.2017.02.006
高效液相色谱法检测乳品中的马尿酸和苯甲酸
Determination of Hippuric Acid and Benzoic Acid in Dairy Products by High Performance Liquid Chromatography
赵贞 1刘春霞 1刘丽君 1岳虹 1万鹏 1李翠枝1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特 010010
- 折叠
摘要
Abstract
A method for determination of hippuric acid and benzoic acid in dairy products by high performance liquid chromatography (HPLC) is described.The protein samples was precipitated by adding lead acetate and centrifuged,and then the analytes were separated on a C18 column with a mobile phase consisting of ammonium acetate and methanol solution and detected with a UV detector at 230 nm.The results showed that hippuric acid and benzoic acid displayed a good linearity relationship in the concentration range of 1-100 mg/kg.The recovery rate was 95.3％-103.6％.The method proved to be simple,accurate and repeatable.关键词
高效液相色谱法/马尿酸/苯甲酸/乳制品Key words
high performance liquid chromatography (HPLC)/hippuric acid/benzoic acid/dairy products分类
轻工纺织引用本文复制引用
赵贞,刘春霞,刘丽君,岳虹,万鹏,李翠枝..高效液相色谱法检测乳品中的马尿酸和苯甲酸[J].乳业科学与技术,2017,40(2):27-29,3.
