四川大学学报（自然科学版）2017，Vol.54Issue(3)：617-622,6.DOI:10.3969/j.issn.0490-6756.2017.03.032
拟南芥AtTR1对AtCPK28和AtCPK32的体外泛素化修饰研究
Investigation on ubiquitination of AtCPK28 and AtCPK32 by AtTR1 in vitro
摘要
Abstract
In order to analyze the relationship between E3 ligase AtTR1 and abiotic stress response related proteins AtCPK28 and AtCPK32,we prepared the prokaryotic expression plasmids pET28a-AtCPK28 and pET28a-AtCPK32,and produced the AtTR1、AtCPK28 and AtCPK32 recombinant proteins in Escherichia coli using RT-PCR and molecular approaches.The recombinant proteins was also purified by Affinity Chromatography.In the presence of ATP、E1、E2 and AtTR1,Poly ubiquitination band was observed in the presence of purified AtCPK28 or AtCPK32 by western blot.The results showed that the AtCPK28 and AtCPK32 were multi-ubiquitylated by AtTR1 in vitro.It indicated that the AtCPK28 and AtCPK32 are potential downstream targets of AtTR1 in the regulation of stress resistance signaling pathways.关键词
拟南芥/AtTR1/AtCPK28/AtCPK32/泛素化Key words
Arabidopsis thialiana/AtTR1/AtCPK28/AtCPK32/Ubiquitination分类
农业科技引用本文复制引用
樊莉娟,陈彩丽,朱旭辉,黄奎,姚润东,王健美..拟南芥AtTR1对AtCPK28和AtCPK32的体外泛素化修饰研究[J].四川大学学报（自然科学版）,2017,54(3):617-622,6.基金项目
国家自然科学基金(31271758) （31271758）
