山东科学2017，Vol.30Issue(3)：17-20,4.DOI:10.3976/j.issn.1002-4026.2017.03.004
HPLC-MS同时测定泽泻中两种泽泻醇的含量
Simultaneous determination of two alisols in Rhizoma Alismatis by HPLC-MS
摘要
Abstract
To improve the quality control of the Rhizoma Alismatis,a HPLC-MS method for determination of the contents of two alisols in Rhizoma Alismatis was established in this paper.Chromatographic conditions were as follows:Halo C18column (2.1 mmx100 mm,2.7 μm),the mobile phase was a mixture of 0.1％ formic acid solution-0.1％ formic acid in acetonitrile,gradient elution,the flow rate was 0.3 mL · min-1,the column temperature was 30 ℃.The conditions of MS included:ESI ion source,detection method with positive ions,mode by MRM,drying temperature was 350 ℃,the flow rate of drying gas was 9 L · min-1,pressure of nebulizer was 241.3 kPa,and the voltage of capillary was 4 000 V.The content of alisol B 23-acetate and alisol A 24-acetate in the samples was 11.4595 μg · g-1and 321.823 9 μg · g-1,respectively.The method is accurate and reproducible to the determination of two alisols in Rhizoma Alismatis.关键词
泽泻/泽泻醇/高效液相-质谱法/含量测定Key words
Rhizoma Alismatis/alisols/HPLC-MS/content determination分类
医药卫生引用本文复制引用
池雨锋,王小明,张玲,林莎莎,蒋海强,周洪雷..HPLC-MS同时测定泽泻中两种泽泻醇的含量[J].山东科学,2017,30(3):17-20,4.基金项目
山东省自然科学基金(ZR2015HM080) （ZR2015HM080）
山东省博士后创新项目专项资金(201403006) （201403006）
山东省科技计划(2013GSF11903) （2013GSF11903）
山东省高校中医药抗病毒协同创新课题(XTCX2014C01-03) （XTCX2014C01-03）
