山东科学2017，Vol.30Issue(3)：21-25,5.DOI:10.3976/j.issn.1002-4026.2017.03.005
HPLC法同时测定有柄石韦中6种有效成分的含量
Simultaneous determination of six active constituents in the Pyrrosia petiolosa (Christ) Ching by HPLC
邵尉 1高德民1
作者信息
- 1. 山东中医药大学药学院,山东济南250355
- 折叠
摘要
Abstract
A HPLC method for simultaneous determination of the contents of six main bioactive constituents (protocatechuic acid,chlorogenic acid,vanillic acid,caffeic acid,mangiferin,and rutin) in the Pyrrosia petiolosa (Christ) Ching was presented.The analysis was performed on SinoChrom ODS-BP C18(4.6 mm × 150 mm,5 μm)column.The six components were separated with gradient elution using mobile phase composed of 0.1％ acetic acid (A)-acetonitrile (B) at a flow rate of 1.0 mL/min.The UV detection wavelength was set at 320 nm.The column temperature was kept at 25℃.Six ingredients showed good linear relationship (R≥0.999 2,n=5),with relative standard deviation values less than 5％.Results reveal that this method is rapid,simple,and accurate,to provide experimental foundation for the quantitative detection and quality control of Medical Pyrrosia petiolosa (Christ) Ching.关键词
有柄石韦/高效液相色谱法/成分/测定Key words
Pyrrosia petiolosa (Christ) Ching/HPLC/ingredient/determination分类
医药卫生引用本文复制引用
邵尉,高德民..HPLC法同时测定有柄石韦中6种有效成分的含量[J].山东科学,2017,30(3):21-25,5.
