药学研究2017，Vol.36Issue(6)：319-321,3.DOI:10.13506/j.cnki.jpr.2017.06.003
低聚原花青素对大鼠肝纤维化氧化损伤的影响
Effects of oligomeric proantho cyanidins on oxidative damage of rat liver fibrosis
摘要
Abstract
Objective To study the effect and mechanism of oligomeric proantho cyanidins on hepatic fibrosis.Methods SD rats were divided randomly into 6 groups:control group,model group,Fuzheng Huayu Capsule group,oligomeric proantho cyanidins group(60,120,240 mg·kg-1).The hepatic fibrosis model was established by intraperitoneal injection of carbon tetrachloride(CCl4).Liver pathology was observed by hematoxylin-eosin (HE) staining.Biochemical method decect Serum alanine aminotransferase(ALT) and aspartate transferase(AST).The contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in liver tissue were determined by enzyme-linked immunosorbent assay(ELISA).Western blot assay was used to detect Sirt1 and Nrf2 protein expression.Results Oligomeric proantho cyanidins(60,120,240 mg·kg-1)significantly inhibited CCl4-induced liver fibrosis in rats.The ALT and AST level in serum,MDA level in liver were significantly reduced,SOD significantly increased.Furthormore,Oligomeric proantho cyanidins were upregulated Sirt1 and Nrf2 protein expression.Conclusion Oligomeric proantho cyanidins have anti-fibrotic effects,its mechanism maybe related to its antioxidant effects.关键词
低聚原花青素/肝纤维化/抗氧化Key words
Oligomeric proantho cyanidins/Liver fibrosis/Anti-oxidation分类
医药卫生引用本文复制引用
陆国辉,李艳茹..低聚原花青素对大鼠肝纤维化氧化损伤的影响[J].药学研究,2017,36(6):319-321,3.基金项目
山东省教育厅高等学校科技计划项目(No.J14LK62) （No.J14LK62）
