药学研究2017，Vol.36Issue(6)：330-333,368,5.DOI:10.13506/j.cnki.jpr.2017.06.006
HPLC-FLD法测定美白类面膜中5种荧光增白剂
Determination of five fluorescent whitening agents in whitening masks by HPLC-FLD mehtod
摘要
Abstract
Objective To establish a method for the determination of five fluorescent whitening agents(FWA85,CBS351,FWA393,FWA378 and FWA368)in whitening masks by HPLC-FLD with fluorescence detector (HPLC-FLD).Methods The HPLC method was performed on an Agilent Zorbax Eclipse XDB-C18(4.6 mm×250 mm,5 μm) column by gradient elution using 20 mmol·L-1 ammonium acetate and methanol as mobile phase,at excitation wavelength of 365 nm and an emission wavelength of 430 nm,which can separate the target analytes successfully.Results The target analytes showed good linearity in the range of 0.05~30 μg·mL-1 with the correlation coefficients(r)greater than 0.99.The limits of detection(S/N=3)were 5.00~13.25 ng·g-1 and the limits of quantification (S/N=10) were 10.04~53.15 ng·g-1 for the target analytes.The feasibility of this method was demonstrated by the determination of FWAs in samples with spiked recoveries.The recoveries were in the range between 92.8% and 108.8%,and the relative standard deviations ranged from 1.8% to 7.3%.Conclusion This method was simple and had high recoveries for the determination of five fluorescent whitening agents in whitening masks.关键词
高效液相色谱/荧光增白剂/美白类面膜Key words
HPLC/Fluorescent whitening agents/Whitening masks分类
化学化工引用本文复制引用
窦月,王乾蕾,田金强,郭星,潘柯伊..HPLC-FLD法测定美白类面膜中5种荧光增白剂[J].药学研究,2017,36(6):330-333,368,5.基金项目
中山市2016年科技支撑计划项目(No.2016K21) （No.2016K21）
