山东医药2017，Vol.57Issue(21)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.21.001
SND1低表达宫颈癌CasKi细胞稳定株的构建及其生物学功能变化
Construction of stable cervical cancer CasKi cell line with low expression of SND1 and its biological function changes
摘要
Abstract
Objective To construct a stable cervical cancer CasKi cell line with low expression of SND1 and detect the effect of SND1 on the biological function of cervical cancer cells. Methods CasKi cells were divided into six groups: the blank control group, negative control group, and interference groups 1-4. The cells in the negative control group and four interference groups were transfected with TRC2-pLKO-puro Vector empty lentivirus and SND1 shRNA(1-4). The stable CasKi cells were screened out by puromycin. Western blotting and RT-PCR were used to detect the expression of SND1 protein and mRNA to confirm the transfection efficiency. The proliferation of CasKi cells was detected by CCK-8 (OD450). The migration distance of CasKi cells was detected by cell Scratch test.Results Cells in the blank control group all die, and some in the negative control group and four interference groups survived, which indicated the stable cells were successfully constructed. Compared with the blank group and negative control group, the mRNA and protein expression levels of SND1 gene decreased in the four interference groups, and significant difference was found between the interference groups 3 and 4 (all P<0.05). Significant difference was found in the OD450 and migration between the blank group, negative control group, and the interference groups 3 and 4 (all P<0.05).Conclusion The stable CasKi cell line with low expression of SND1 is successfully established, and its proliferation and migration abilities decrease.关键词
子宫颈肿瘤/SND1蛋白/CasKi细胞/细胞增殖/细胞迁移Key words
cervical neoplasms/SND1 protein/CasKi cells/cell proliferation/cell migration分类
医药卫生引用本文复制引用
孟浩,付晓,张春燕,辛灵彪,任媛媛,杨洁,何津岩..SND1低表达宫颈癌CasKi细胞稳定株的构建及其生物学功能变化[J].山东医药,2017,57(21):1-4,4.基金项目
国家杰出青年基金资助项目(31125012) （31125012）
教育部"创新团队发展计划"(IRT13085) （IRT13085）
国家自然科学基金资助项目(31670759、31370749、31501056) （31670759、31370749、31501056）
天津市自然科学基金项目(16JCQNJC09000). （16JCQNJC09000）
